The extracellular matrix protects Pseudomonas aeruginosa biofilms by limiting the penetration of tobramycin.
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...aeruginosa biofilm, tobramycin was shown to exhibit delayed and reduced diffusion in vitro (41, 42)....
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...Therefore, the physical and physiological 30 attributes of the biofilm growth environment protect resident cells from the lethal action 31 of antimicrobials (Costerton et al., 1999)....
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...432 433 MBEC viability assay 434 Biofilms were cultivated in the Calgary Biofilm Device (MBEC™ Physiology and 435 Genetics Assay, Innovotech Inc.) as described previously (Ceri et al., 1999; Harrison et 436 al., 2010)....
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"The extracellular matrix protects P..." refers background in this paper
...Moreover, the 28 antibiotic susceptibility of biofilm bacteria returns upon dispersion of the biofilm cells 29 back to the planktonic state (Stewart, 2002)....
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...…1992; Dunne et al., 1993; Yasuda et al., 1993; Kumon et 60 al., 1994; Suci et al., 1994; Shigeta et al., 1997; Vrany et al., 1997; Anderl et al., 2000; 61 Stone et al., 2002; Zheng and Stewart, 2002; Walters et al., 2003; Jefferson et al., 2005; 62 Singh et al., 2010; Ortiz-Perez et al., 2011)....
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...While heightened tolerance is clearly due to multiple mechanisms 284 (Stewart, 2002), here we showed that non-mucoid P. aeruginosa biofilms protect 285 resident cells by limiting the penetration of tobramycin via ionic interactions with the 286 biofilm....
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...While heightened tolerance is clearly due to multiple mechanisms 284 (Stewart, 2002), here we showed that non-mucoid P....
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