Journal ArticleDOI
The First Analogues of LNA (Locked Nucleic Acids): Phosphorothioate-LNA and 2′-Thio-LNA
Ravindra Kumar,Sanjay K. Singh,Alexei A. Koshkin,Vivek K. Rajwanshi,Michael Meldgaard,Jesper Wengel +5 more
Reads0
Chats0
TLDR
A 9-mer phosphorothioate-LNA with three LNA thymine monomers (1, X = O, Y = S, S, Base = thymin-1-yl) and nine-mer LNA with one, three or five 2-thio-Lna monomers were shown to recognize both complementary DNA and RNA with thermal affinities comparable to those of parent LNA as mentioned in this paper.Abstract:
LNA (Locked Nucleic Acids, 1, X = O, Y = O) is a novel oligonucleotide analogue capable of recognizing complementary DNA and RNA with unprecedented thermal affinities. Synthesis of the first chemically modified LNA analogues is reported. A 9-mer phosphorothioate-LNA containing three LNA thymine monomers (1, X = O, Y = S, Base = thymin-1-yl) and 9-mer LNAs containing one, three or five 2'-thio-LNA monomers (1, X = S, Y = O, Base = uracil-1-yl) were able to recognize both complementary DNA and RNA with thermal affinities comparable to those of parent LNA.read more
Citations
More filters
Journal ArticleDOI
Application of locked nucleic acids to improve aptamer in vivo stability and targeting function
Kathrin Schmidt,Sandra Borkowski,Jens Kurreck,Andrew Stephens,Rolf Bald,Maren Hecht,Matthias Friebe,Ludger Dinkelborg,Volker A. Erdmann +8 more
TL;DR: The strategy to introduce LNA modifications after the selection procedure is likely to be generally applicable to improve the in vivo stability of aptamers without compromising their binding properties.
Journal ArticleDOI
Non-coding RNAs: the riddle of the transcriptome and their perspectives in cancer.
TL;DR: Results reinforce the hypothesis, that ncRNAs constitute therapeutic targets, and point out their clinical potential as stratification markers, to discuss their clinical implicit as prognostic diagnostic indicators, biomarkers, and therapeutic targets.
Patent
POSITIONALLY MODIFIED siRNA CONSTRUCTS
Balkrishen Bhat,Eric E. Swayze,Thazha P. Prakash,Charles R. Allerson,Prasad Dande,Richard H. Griffey +5 more
TL;DR: In this article, the authors presented a method for the synthesis of oligomeric compounds having sufficient complementarity to hybridize to a nucleic acid target and methods for their use in modulating gene expression.
Patent
Selective antisense compounds and uses thereof
TL;DR: In this article, the authors provide oligomeric compounds for hybridizing to a complementary nucleic acid, including but not limited, to nucleic acids in a cell, which results in modulation of the amount, activity, or expression of the target nucleic acyclic acid in the cell.
Journal ArticleDOI
NMR solution structure of a parallel LNA quadruplex.
Antonio Randazzo,Veronica Esposito,Oliver Ohlenschläger,Ramadurai Ramachandran,Luciano Mayol +4 more
TL;DR: The structural features of the LNA quadruplex reported here may open new perspectives for the biological application of LNAs as novel versatile tools to design aptamer or catalyst oligonucleotides.
References
More filters
Journal ArticleDOI
The first analogues of LNA (locked nucleic acids): phosphorothioate-LNA and 2'-thio-LNA.
Ravindra Kumar,Sanjay K. Singh,Alexei A. Koshkin,Vivek K. Rajwanshi,Michael Meldgaard,Jesper Wengel +5 more
TL;DR: A 9-mer phosphorothioate-LNA containing three LNA thymine monomers were able to recognize both complementary DNA and RNA with thermal affinities comparable to those of parent LNA.
Related Papers (5)
Potent and nontoxic antisense oligonucleotides containing locked nucleic acids
Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA
Dwaine A. Braasch,David R. Corey +1 more