The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential * † ‡
TL;DR: Sperm from fertile men exhibited significantly higher binding capacity to hemizonae compared with sperm from men who had fertilization failure during in vitro fertilization (IVF) treatment, demonstrating that the HZA may be a useful diagnostic tool in male infertility evaluations.
About: This article is published in Fertility and Sterility.The article was published on 1989-02-01. It has received 390 citations till now. The article focuses on the topics: Sperm-Ovum Interactions & Sperm.
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01 Jan 2010
2,199 citations
TL;DR: Along with being an epithelial differentiation marker, glycodelin appears to play a role in glandular morphogenesis, as transfection of gly codelin cDNA into a glyCodelin-negative breast cancer cells resulted in formation of gland-like structures, restricted proliferation, and induction of other epithelial markers.
Abstract: Glycodelin is a glycoprotein that belongs to the lipocalin superfamily. Depending on glycosylation, glycodelin appears in various isoforms. In the uterus, glycodelin-A is the major progesterone-regulated glycoprotein secreted into uterine luminal cavity by secretory/decidualized endometrial glands. The other tissues expressing glycodelin include fallopian tubes, ovary, breast, seminal vesicle, bone marrow, and eccrine glands. Glycodelin-A potently and dose-dependently inhibits human sperm-egg binding, whereas differently glycosylated glycodelin-S from seminal plasma has no such effect. Absence of contraceptive glycodelin-A in the uterus during periovulatory midcycle is consistent with an open “fertile window.” Glycodelin induced by local or systemic administration of progestogens may potentially reduce the fertilizing capacity of sperm in any phase of the menstrual cycle. Glycodelin also has immunosuppressive activity. Its high concentration at the fetomaternal interface may contribute to protection of th...
252 citations
Cites background from "The hemizona assay (HZA): developme..."
...Data about human sperm-egg binding can be obtained using an in vitro system, known as the hemizona assay (135)....
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TL;DR: Analysis by high-performance liquid chromatography/mass spectrometry shows the presence of AEA, PEA, and OEA in human seminal plasma, mid-cycle oviductal fluid, follicular fluid, amniotic fluid, milk, and fluids from malignant ovarian cysts, suggesting that NAEs in human reproductive fluids may help regulate multiple physiological and pathological processes in the reproductive system and imply that exogenous cannabinoids delivered by marijuana smoke might impact these processes.
226 citations
Cites background from "The hemizona assay (HZA): developme..."
...Nevertheless, fertilizing potential of human sperm can be determined on the basis of tight binding of capacitated sperm to the zona pellucida in the hemizona assay (Burkman et al., 1988; Burkman, 1995; Franken et al., 1989)....
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TL;DR: It is suggested that consistent with quality standards that should be required for cryoprotectant media and because of the superior quality of the egg yolk-free extender, a defined soybean lecithin-containing diluter might be the better choice as a semen extender in the future.
217 citations
Additional excerpts
...The HZA was performed as previously described for human and bovine gametes [11,12]....
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TL;DR: Results of in vitro fertilization can be used to evaluate tests of human sperm function and Logistic regression analysis is a powerful method for determining which groups of sperm characteristics are independently related to fertilization rates.
208 citations
References
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TL;DR: In this article, the authors conducted a prospective study in women with bilateral tubal damage to determine whether there is a prognostic value in the percentage normal sperm morphologic features in a human in vitro fertilization (IVF) program.
1,153 citations
TL;DR: By evaluating sperm morphology with the proposed strict criteria, its predictive value in in vitro fertilization is enhanced.
1,095 citations
TL;DR: The results suggest that zona-free hamster ova can be substituted for human ova in the preliminary assessment of the fertilizing capacity of human spermatozoa.
Abstract: The zona-free hamster ovum was evaluated as a substitute for human o va in the assessment of the fertilizing capacity of human spermatozoa. Zona-intact ova completely resisted sperm penetration. Using nonpreincu bated spermatozoa sperm penetration of zona-free ova began 4-5 hours after insemination. However when spermatozoa were preincubated in a modified Krebs-Ringer solution for 4 hours sperm penetration began within 1 hour. There is some evidence that this is associated with the completion of sperm capacitation and the acrosome reaction. The results suggest that zona-free hamster ova can be substituted for human ova in the preliminary assessment of the fertilizing capacity of human spermatozoa.
871 citations
01 Jan 1981
TL;DR: This chapter will discuss how mammalian spermatozoa prepare themselves for fertilization and how the spermatozosa and eggs interact during fertilization.
Abstract: The spermatozoa of most invertebrates (e.g., sea urchins) and nonmammalian vertebrates (e.g., fishes and amphibians) have full capacity to fertilize eggs upon leaving the testis. Testicular spermatozoa of mammals, on the other hand, do not possess the ability to do so. Their fertilizing capacity develops as they pass through the epididymis (Young, 1931; Nishikawa and Waide, 1952; Blandau and Rumery, 1964; Bedford, 1966; Orgebin-Crist, 1967). This process, apparently “unique” to mammals, is referred to as the epididymal maturation of spermatozoa. Even after their maturation, however, spermatozoa require an additional phase of maturation or capacitation within the female genital tract before they are able to fertilize eggs (Austin, 1951, 1952; Chang, 1951a). Thus, epididymal maturation and capacitation are two extra steps that mammaliam spermatozoa must take before they can effect fertilization. In this chapter, I will discuss how mammalian spermatozoa prepare themselves for fertilization and how the spermatozoa and eggs interact during fertilization. The process and mechanisms of sperm transport in the female genital tract will not be dealt with extensively here. Readers are referred to Bishop (1961, 1969), Blandau (1969), Bedford (1970b, 1972b), Thibault (1972, 1973a), Zamboni (1972), Blandau and Gaddum-Rosse (1974), Hafez and Thibault (1975), Overstreet and Katz (1977), Overstreet and Cooper (1978, 1979b), Overstreet et al. (1978), Shalgi and Kraicer(1978), Cooper et al. (1979) and Hunter (1975, 1980). The rejection or elimination of extra spermatozoa by the fertilized egg, one of the most fascinating events in fertilization, will not be discussed here. Instead, readers are referred to the chapter by Dr. Wolf in this volume. The physiology of egg activation has been described and discussed to some extent by Gwatkin (1977) and Yanagimachi (1978a). There are numerous reviews dealing with general aspects of mammalian fertilization. The following are recommended to aid in grasping the outline of mammalian fertilization: Austin and Bishop (1957), Austin and Walton (1960), Austin (1961, 1968), Blandau (1961), Piko (1969), Thibault (1969), Bedford (1970a,b, 1972b), Gwatkin (1976, 1977), Yanagimachi (1977, 1978a), Bedford and Cooper (1978) and Hunter (1980).
773 citations
Book•
01 Jan 1970
TL;DR: In this article, statistics with applications to the biological and health sciences, Statistics with application to the Biological and Health Sciences (B&H), Statistics with applications in the field of biology and medicine.
Abstract: Statistics with applications to the biological and health sciences , Statistics with applications to the biological and health sciences , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
496 citations