The heterogeneity of megakaryocytes and platelets and implications for ex vivo platelet generation.
Reads0
Chats0
TLDR
The functional and developmental heterogeneity of megakaryocytes (MKs) and its potential link to the heterogeneity of platelets has been studied in this article, and the implications of these findings while focusing on the ex vivo generation of platelet from human pluripotent stem cells.Abstract:
Platelets, the chief effector of hemostasis, are small anucleate blood cells generated from megakaryocytes (MKs), and the defects in platelet production or function lead to a variety of bleeding complications. Emerging evidence indicates that MKs and platelets are much more diverse than previously appreciated and involved in many physiological and pathological processes besides hemostasis, such as innate and adaptive immune responses, angiogenesis, and tumor metastasis, while the ontogenic variations in MK and platelet function have also become a focus in the field. However, whether MKs and platelets fulfill these distinct functions by utilizing distinct subpopulations remains poorly understood. New studies aimed at deciphering the MK transcriptome at the single-cell level have provided some key insights into the functional heterogeneity of MKs. In this review, we will discuss some of the recent discoveries of functional and developmental heterogeneity of MKs and its potential link to the heterogeneity of platelets. We will also discuss the implications of these findings while focusing on the ex vivo generation of platelets from human pluripotent stem cells. The improved understanding of the heterogeneity underlying human MK development and platelet production should open new avenues for future platelet regeneration and clinical treatment of related diseases.read more
Citations
More filters
Journal ArticleDOI
Illustrated State‐of‐the‐Art Capsules of the ISTH 2022 Congress
Robert A. S. Ariëns,Beverley J. Hunt,E.O. Agbani,Josefin Ahnström,Robert Ahrends,Raza Alikhan,Alice Assinger,Zsuzsa Bagoly,Alessandra Balduini,Elena Barbon,Christopher D. Barrett,P. Batty,Jorge Carneiro,Wee-Shian Chan,Moniek P.M. de Maat,Kerstin de Wit,Cécile V. Denis,Martin Elvis,Renee Eslick,Hongxia Fu,Catherine P.M. Hayward,Benoît Ho-Tin-Noé,Frederikus A. Klok,Riten Kumar,Karin Leiderman,Rustem I. Litvinov,Nigel Mackman,Zoe McQuilten,Matthew D. Neal,William A E Parker,Roger J. S. Preston,Julie Rayes,Alireza R. Rezaie,Lara N. Roberts,Bianca Rocca,Susan Shapiro,Deborah M. Siegal,Lirlândia P. Sousa,Katsue Suzuki-Inoue,Tahira Zafar,Jiaxi Zhou +40 more
TL;DR: The ISTH London 2022 Congress is the first held (mostly) face‐to‐face again since the COVID‐19 pandemic took the world by surprise in 2020, and this year’s in‐person format will allow the ever‐so‐important and quintessential creativity and networking to flow again.
Journal ArticleDOI
Emerging Technologies for Understanding Platelet Diversity
TL;DR: In this article , a review of platelet diversity with implications for the roles of platelets in hemostasis and thrombosis and identifies advanced technologies set to provide new insights.
Journal ArticleDOI
Anti-inflammatory activity of platelet-rich plasma treatment in the inflammation management of knee osteoarthritis: experimental study
Journal ArticleDOI
A Novel Megakaryocyte Subpopulation Poised to Exert the Function of HSC Niche as Possible Driver of Myelofibrosis
TL;DR: In this article, it was shown that platelets are released in the circulation by mature megakaryocytes generated by hematopoietic stem cells by giving rise to lineage-restricted progenitor cells, which undergo a process of terminal maturation.
Journal ArticleDOI
A high-efficiency differential expression method for cancer heterogeneity using large-scale single-cell RNA-sequencing data
Xin Yuan,Shuangge Ma,Botao Fa,Ting Wei,Yanran Ma,Yifan Yang,Wenwen Lv,Junke Zheng,Guo-Rui Chen,Jing Sun,Zhangsheng Yu +10 more
TL;DR: HEART as discussed by the authors is a statistical combination test that can detect DE genes with various sources of differences beyond mean expression changes, and it has high accuracy (F 1 score > 0.75) and brilliant computational efficiency (less than 2 min) on multiple simulation datasets.
References
More filters
Journal ArticleDOI
Platelets and the immune continuum
TL;DR: The mechanisms by which platelets contribute to immunity are discussed: these small cells are more immunologically savvy than the authors once thought.
Journal ArticleDOI
Single-Cell Transcriptomics of Human and Mouse Lung Cancers Reveals Conserved Myeloid Populations across Individuals and Species.
Rapolas Zilionis,Rapolas Zilionis,Camilla Engblom,Christina Pfirschke,Virginia Savova,David Zemmour,Hatice D. Saatcioglu,Indira Krishnan,Indira Krishnan,Giorgia Maroni,Giorgia Maroni,Giorgia Maroni,Claire V. Meyerovitz,Clara M. Kerwin,Sun Choi,William G. Richards,Assunta De Rienzo,Daniel G. Tenen,Daniel G. Tenen,Raphael Bueno,Elena Levantini,Elena Levantini,Elena Levantini,Mikael J. Pittet,Allon M. Klein +24 more
TL;DR: The lung TIM landscape is determined and sets the stage for future investigations into the potential of TIMs as immunotherapy targets by using single-cell RNA sequencing to map TIMs in non-small-cell lung cancer patients.
Journal ArticleDOI
Dynamic Visualization of Thrombopoiesis Within Bone Marrow
Tobias Junt,Harald Schulze,Zhao Chen,Steffen Massberg,Tobias Goerge,Andreas Krueger,Denisa D. Wagner,Thomas Graf,Joseph E. Italiano,Ramesh A. Shivdasani,Ulrich H. von Andrian +10 more
TL;DR: The use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice confirms the concept of proplatelet formation in vivo and is consistent with the possibility that blood flow–induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis.
Journal ArticleDOI
The incredible journey: From megakaryocyte development to platelet formation
Kellie R. Machlus,Kellie R. Machlus,Joseph E. Italiano,Joseph E. Italiano,Joseph E. Italiano +4 more
TL;DR: Understanding how large progenitor cells in the bone marrow called megakaryocytes release platelets occurs is an active area of research with important clinical applications.
Journal ArticleDOI
The complex transcriptional landscape of the anucleate human platelet
Paul F. Bray,Steven E. McKenzie,Leonard C. Edelstein,Srikanth Nagalla,Kathleen Delgrosso,Adam Ertel,Joan Kupper,Yi Jing,Eric Londin,Phillipe Loher,Huang Wen Chen,Paolo Fortina,Isidore Rigoutsos +12 more
TL;DR: Deep-sequence RNA from leukocyte-depleted platelets is deep-sequenced to capture the complex profile of all expressed transcripts, revealing diverse classes of non-coding RNAs, including: pervasive antisense transcripts to protein-c coding loci; numerous, previously unreported and abundant microRNAs; retrotransposons; and thousands of novel un-annotated long and short intronic transcripts, an intriguing finding considering the anucleate nature of platelets.