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The Histological Examination of Mummified Material
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TLDR
Tissue from Egyptian mummy material is extremely brittle; hence it was handled in perforated glass tubes during processing, which allowed regular sectioning and staining to be done successfully.Abstract:
Tissue from Egyptian mummy material is extremely brittle; hence it was handled in perforated glass tubes during processing. The first (softening) fluid consisted of 96% ethyl alcohol, 30 vol; 1% aqueous formalin, 50 vol; 5% aqueous Na2CO3, 20 vol. It was used in a fluid to tissue volume ratio of 100:1 and allowed to act overnight. A special dehydrating sequence: 80% alcohol, 3-6 hr; 8% phenol in 96% alcohol, and absolute alcohol followed by 3 changes of amyl acetate, 6-18 hr each; 3 changes of 1 % celloidin in methyl benzoate, 24 hr each; then through benzene and embedding in paraffin completed the special technic. This allowed regular sectioning and staining to be done successfully.read more
Citations
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Molecular cloning of Ancient Egyptian mummy DNA
TL;DR: Analysis of 23 mummies investigated for DNA content show that substantial pieces of mummy DNA can be cloned and that the DNA fragments seem to contain little or no modifications introduced postmortem.
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Paleohistopathology of bone: A new approach to the study of ancient diseases
TL;DR: As a reliable diagnosis is the basis not only of the study of case reports but also of the etiology and epidemiology of diseases in ancient populations, paleopathologists would be well-advised to employ histological analysis for their research, to avoid false diagnoses.
Journal ArticleDOI
Manual preparation of ground sections for the microscopy of natural bone tissue: update and modification of Frost's ‘rapid manual method’
TL;DR: A long existing technique for the manual preparation of ground sections was brushed up and tested, and a few modifications are proposed which further improve the feasibility of the technique, and which also make it very suitable for less well-preserved, inhumed, and even cremated osteoarchaeological/forensic remains.
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Preservation of DNA in ancient Egyptian mummies
TL;DR: The preservation of nucleic acids in this specimen suggests that applying recombinant DNA techniques to the study of ancient mummified tissues might prove to be a fruitful future area of research.
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Contributions of Chemical Dietary Reconstruction to the Assessment of Adaptation by Ancient Highland Immigrants (Alto Ramirez) to Coastal Conditions at Pisagua, North Chile
Arthur C. Aufderheide,Marc A. Kelley,Mario A. Rivera,Luz Gray,Larry L. Tieszen,Elysha Iversen,H.Roy Krouse,Alvaro Carevic +7 more
TL;DR: In this paper, the authors investigated the adaptation of the Alto Ramirez cultural group in the lower valleys of northern Chile to the coastal environment by using chemical dietary reconstruction and anatomic observations.
References
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A Report upon the Pathological Condition of the Aorta of King Menephtah, traditionally regarded as the Pharaoh of the Exodus.
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