The host plant Pinus pinaster exerts specific effects on phosphate efflux and polyphosphate metabolism of the ectomycorrhizal fungus Hebeloma cylindrosporum: a radiotracer, cytological staining and 31P NMR spectroscopy study
Margarita Torres-Aquino,Adeline Becquer,Christine Le Guernevé,Julien Louche,Laurie Amenc,Siobhan Staunton,Hervé Quiquampoix,Claude Plassard +7 more
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The results support the existence of specific host plant effects on fungal P metabolism able to provide P in the apoplast of ectomycorrhizal roots, and specifically increased the proportion of short-chain polyP in the interacting mycelia.Abstract:
Ectomycorrhizal (ECM) association can improve plant phosphorus (P) nutrition. Polyphosphates (polyP), synthesized in distant fungal cells after P uptake may contribute to P supply from the fungus to the host plant if they are hydrolyzed to phosphate in ECM roots then transferred to the host plant when required. In this study, we addressed this hypothesis for the ECM fungus Hebeloma cylindrosporum grown in vitro and incubated without plant or with host (Pinus pinaster) and non-host (Zea mays) plants, using an experimental system simulating the symbiotic interface. We used 32P labelling to quantify P accumulation and P efflux and in vivo and in vitro NMR spectroscopy and cytological staining to follow the fate of fungal polyP. Phosphate supply triggered a massive P accumulation as newly synthesized long-chain polyP in H. cylindrosporum if previously grown under P-deficient conditions. P efflux from H. cylindrosporum towards the roots was stimulated by both host and non-host plants. However, the host plant enhanced 32P release compared to the non-host plant and specifically increased the proportion of short-chain polyP in the interacting mycelia. These results support the existence of specific host-plant effects on fungal P metabolism able to provide P in the apoplast of ectomycorrhizal roots.read more
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Journal ArticleDOI
Nitrogen and phosphate metabolism in ectomycorrhizas.
Uwe Nehls,Claude Plassard +1 more
TL;DR: In this article, the authors present a review of the entire pathway from nutrient mobilization, import and local storage, distribution within the mycelium and export at the plant-fungus interface.
Book ChapterDOI
The ectomycorrhizal contribution to tree nutrition
Adeline Becquer,Adeline Becquer,Carmen Guerrero-Galán,Janice L. Eibensteiner,Gabriella Houdinet,Heike Bücking,Sabine Zimmermann,Kevin Garcia +7 more
TL;DR: The molecular mechanisms driving the acquisition, translocation and release of water and nutrients in ectomycorrhizal systems are highlighted, particularly on the transport of macronutrients, including nitrogen, phosphorus, potassium, sulphur and calcium, micronutrient, and water by the symbiotic partner.
Journal ArticleDOI
Harnessing Soil Microbes to Improve Plant Phosphate Efficiency in Cropping Systems
Arjun Kafle,Kevin R. Cope,Rachel Raths,Jaya Krishna Yakha,Senthil Subramanian,Heike Bücking,Kevin Garcia +6 more
TL;DR: This review highlights recent advances in the understanding and utilization of soil microbes to both solubilize inorganic phosphate from insoluble forms and allocate it directly to crop plants, and focuses on arbuscular mycorrhizal fungi, ectomycorrhizer fungi, and phosphate-solubilizing bacteria.
Journal ArticleDOI
The Hebeloma cylindrosporum HcPT2 Pi transporter plays a key role in ectomycorrhizal symbiosis.
Adeline Becquer,Kevin Garcia,Kevin Garcia,Laurie Amenc,Camille Rivard,Camille Rivard,Jeanne Doré,Carlos Trives-Segura,Wojciech Szponarski,Sylvie Russet,Yoan Baeza,Benedikt Lassalle-Kaiser,Sabine Zimmermann,Claude Plassard +13 more
TL;DR: The results reveal that HcPT2 plays an important role in ectomycorrhizal symbiosis, affecting both Pi influx in the mycelium and efflux towards roots under the control of P. pinaster.
References
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Christine Rausch,Pierre Daram,Silvia Brunner,Jan Jansa,Maryse Laloi,Georg Leggewie,Nikolaus Amrhein,Marcel Bucher +7 more
TL;DR: RNA localization and reporter gene expression indicated expression of StPT3 in root sectors where mycorrhizal structures are formed, suggesting that the mutualistic symbiosis evolved by genetic rearrangements in the St PT3 promoter.
Journal ArticleDOI
A Secreted Effector Protein of Laccaria bicolor Is Required for Symbiosis Development
Jonathan M. Plett,Minna Kemppainen,Shiv D. Kale,Annegret Kohler,Valérie Legué,Annick Brun,Brett M. Tyler,Alejandro Guillermo Pardo,Francis Martin +8 more
TL;DR: It is demonstrated that MYCORRHIZAL iNDUCED SMALL SECRETED PROTEin 7 (MiSSP7), the most highly symbiosis-upregulated gene from the ectomycorrhizal fungus Laccaria bicolor, encodes an effector protein indispensible for the establishment of mutualism.
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