The Ingestion of Proteins and Colloidal Materials by Columnar Absorptive Cells of the Small Intestine in Suckling Rats and Mice
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"The Ingestion of Proteins and Collo..." refers methods in this paper
...For electron microscopy, the tissues were fixed approximately 1 hour at room temperature in buffered 1 per cent osmium tetroxide (Palade, 1952) containing 3.5 per cent sucrose, dehydrated rapidly in graded ethyl alcohol-water mixtures, imbedded in a 6:1 mixture of butyl and methyl methacrylate…...
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...For electron microscopy, the tissues were fixed approximately 1 hour at room temperature in buffered 1 per cent osmium tetroxide (Palade, 1952) containing 3....
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...For electron microscopy, the tissues were fixed approximately 1 hour at room temperature in buffered 1 per cent osmium tetroxide (Palade, 1952) containing 3.5 per cent sucrose, dehydrated rapidly in graded ethyl alcohol-water mixtures, imbedded in a 6:1 mixture of butyl and methyl methacrylate polymerized with benzoyl peroxide at 60°C., sectioned with glass knives in a Porter-Blum microtome (Porter and Blum, 1953), and examined in an RCA model EMU 2E electron microscope with a compensated objective lens and a 40 micron objective aperture. Sections 1 to 4 microns thick, prepared in the same way, were examined by phase contrast microscopy. For staining with fluorescent antibody, the tissues were freeze-dried, imbedded in butyl methacrylate in vacuo, polymerized as described above, sectioned at 2 to 3 microns using glass knives in a Porter-Bluln microtome, transferred to gl~tss slides by floating on water, dried at 37°C., and immersed in ethylene dichloride for 1 hour to remove the plastic prior to staining. Sections prepared in this manner were used also for histological staining. Fluorescent antibodies against bovine gamma globulin and ovalbumin, generously provided by Dr. Jack Davies, were prepared by injecting rabbits subcutaneously with saline suspensions of the proteins emulsified with the adjuvant recommended by Moloney and Coval (1955). After several weekly injections of...
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"The Ingestion of Proteins and Collo..." refers methods in this paper
...The globulin fraction was conjugated with fluorescein isocyanate according to the method of Coons and Kaplan (1950), and absorbed with rabbit liver powder to prevent non-specific staining (Coons, Leduc, and Connolly, 1955)....
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