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Journal ArticleDOI

The locomotion of fibroblasts in culture I. Movements of the leading edge

01 Mar 1970-Experimental Cell Research (Academic Press)-Vol. 59, Iss: 3, pp 393-398
TL;DR: During the locomotion on a plane surface of fibroblast-like cells from embryonic chick heart and neonatal mouse muscle, any point on the leading edge undergoes a repetitive protrusion and withdrawal, covering about 5 μm which is quantitatively very similar in the two kinds of cell.
About: This article is published in Experimental Cell Research.The article was published on 1970-03-01. It has received 398 citations till now. The article focuses on the topics: Leading edge.
Citations
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Journal ArticleDOI
TL;DR: Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.
Abstract: Taxol, a potent inhibitor of human HeLa and mouse fibroblast cell replication, blocked cells in the G2 and M phase of the cell cycle and stabilized cytoplasmic microtubules. The cytoplasmic microtubules of taxol-treated cells were visualized by transmission electron microscopy and indirect immunofluorescence microscopy. More than 90% of the cells treated with 10 micro M taxol for 22 hr at 37 degrees C displayed bundles of microtubules that appeared to radiate from a common site (or sites), in addition to their cytoplasmic microtubules. Untreated cells that were kept in the cold (4 degrees C) for 16 hr lost their microtubules, whereas cells that were pretreated with taxol for 22 hr at 37 degrees C continued to display their microtubules and bundles of microtubules in the cold. Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.

1,910 citations

Journal ArticleDOI
06 Feb 1997-Nature
TL;DR: It is shown that changes in cell migration speed resulting from three separate variables—substratum ligand level, cell integrin expression level, and integrin–ligand binding affinity—are all quantitatively predictable through the changes they cause in a single unifying parameter: short-term cell– substratum adhesion strength.
Abstract: Migration of cells in higher organisms is mediated by adhesion receptors, such as integrins, that link the cell to extracellular-matrix ligands, transmitting forces and signals necessary for locomotion. Whether cells will migrate or not on a given substratum, and also their speed, depends on several variables related to integrin-ligand interactions, including ligand levels, integrin levels, and integrin-ligand binding affinities. These and other factors affect the way molecular systems integrate to effect and regulate cell migration. Here we show that changes in cell migration speed resulting from three separate variables-substratum ligand level, cell integrin expression level, and integrin-ligand binding affinity-are all quantitatively predictable through the changes they cause in a single unifying parameter: short-term cell-substratum adhesion strength. This finding is consistent with predictions of a mathematical model for cell migration. The ligand concentration promoting maximum migration speed decreases reciprocally as integrin expression increases. Increases in integrin-ligand affinity similarly result in maximal migration at reciprocally lower ligand concentrations. The maximum speed attainable, however, remains unchanged as ligand concentration, integrin expression, or integrin-ligand affinity vary, suggesting that integrin coupling with intracellular motors remains unaltered.

1,425 citations

Journal ArticleDOI
TL;DR: It is found that the propulsive thrust for fibroblast locomotion is imparted to the substratum within 15 micrometers of the leading edge, demonstrating that the lamellipodium of the fibro Blast is able to generate intense traction stress.

1,287 citations

Journal ArticleDOI
TL;DR: It is shown that cell-surface attachment is not sensitive to pattern density, whereas the formation of stable focal adhesions and persistent spreading is, and a critical RGD density is essential for the establishment of mature and stable integrin adhesion, which, in turn, induce efficient cell spreading and formation of focal adhesion dynamics.

880 citations

References
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Book
01 Jan 1956
TL;DR: This is the revision of the classic text in the field, adding two new chapters and thoroughly updating all others as discussed by the authors, and the original structure is retained, and the book continues to serve as a combined text/reference.
Abstract: This is the revision of the classic text in the field, adding two new chapters and thoroughly updating all others. The original structure is retained, and the book continues to serve as a combined text/reference.

35,552 citations

Journal ArticleDOI
TL;DR: It is suggested that the slowing of fibroblasts by contact with others is mainly to be ascribed to the effects of adhesions between the cells; and the acceleration by change of contact, to the effect of development or rupture of such adhesion.

497 citations

Journal ArticleDOI
17 May 1969-Nature
TL;DR: Observations of fibroblasts by time lapse and still microphotography clarify the nature of the “ruffling” phenomenon, and suggest a mechanism for locomotion and contact inhibition.
Abstract: Observations of fibroblasts by time lapse and still microphotography clarify the nature of the “ruffling” phenomenon, and suggest a mechanism for locomotion and contact inhibition.

180 citations