The microRNAs of Caenorhabditis elegans
Citations
32,946 citations
Cites background from "The microRNAs of Caenorhabditis ele..."
...Fourth, miRNA sequences are nearly always con-metazoan miRNAs (Lewis et al., 2003; Lim et al., 2003a). served in related organisms, whereas endogenous(4) When predicting targets of mammalian miRNAs, resiRNA sequences are rarely conserved....
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...…the multitude of other 21 to 24 ntmiRNA genes; Lim et al., 2003b), C. elegans (103–120 RNAs found in plants sometimes complicated their initialgenes; Lim et al., 2003a; Ohler et al., 2004), and Drosophclassification (Llave et al., 2002a; Mette et al., 2002; Parkila (96–124 genes; Lai et al.,…...
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...…RNAs from plants has also revealedber of miRNA genes in the genomes of human (200–255 miRNAs, although the multitude of other 21 to 24 ntmiRNA genes; Lim et al., 2003b), C. elegans (103–120 RNAs found in plants sometimes complicated their initialgenes; Lim et al., 2003a; Ohler et al., 2004),…...
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...Bashirullah et al., 2003; Lim et al., 2003a)....
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...The miRNAs* (blue) are also shown in cases where they have been experimentally identified (Lim et al., 2003a)....
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18,036 citations
Cites background from "The microRNAs of Caenorhabditis ele..."
...This assertion dovetailed nicely with previous reports that the 5′ region is the most conserved portion of the metazoan miRNAs (Lim et al., 2003) and the 5′ region of certain Drosophila miRNAs perfectly matches 3′-UTR elements that mediate mRNA decay and translational repression (Lai, 2002), as…...
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...This assertion dovetailed nicely with previous reports that the 5′ region is the most conserved portion of the metazoan miRNAs (Lim et al., 2003) and the 5′ region of certain Drosophila miRNAs perfectly matches 3′-UTR elements that mediate mRNA decay and translational repression (Lai, 2002), as well as subsequent experiments showing that miRNA-like regulation was most sensitive to nucleotide substitutions that disrupt seed pairing (Doench and Sharp, 2004; Kloosterman et al....
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...An answer to this question becomes apparent when considering the other let-7 family members in worms, which include let-7, miR-48, miR-84, and miR-241, all of which have the same seed region but differ in their remaining sequence (Lau et al., 2001; Lim et al., 2003)....
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11,624 citations
Additional excerpts
...the same seeds (Lim et al., 2003) but are...
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9,986 citations
6,282 citations
Cites background from "The microRNAs of Caenorhabditis ele..."
...In addition to experimental approaches, bioinformatic predictions have helped to identify novel miRNAs in various organisms, mostly on the basis of pre-miRNA hairpin structures and sequence conservation throughout evolutio...
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References
63,427 citations
"The microRNAs of Caenorhabditis ele..." refers methods in this paper
...To create RNA consensus structures, alidot and pfrali combine a Clustal alignment (Thompson et al. 1994) of a pair of sequences with either the minimum free energy structures of these sequences (alidot) derived using the Zuker algorithm (Zuker 1994) or the base...
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...To create RNA consensus structures, alidot and pfrali combine a Clustal alignment (Thompson et al. 1994) of a pair of sequences with either the minimum free energy structures of these sequences (alidot) derived using the Zuker algorithm (Zuker 1994) or the base pairing probability matrices of these…...
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...briggsae secondary structure, as generated by using ClustalW (Thompson et al. 1994) and Alidot (Hofacker and Stadler 1999)....
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22,269 citations
11,932 citations
"The microRNAs of Caenorhabditis ele..." refers background in this paper
...Given the known roles of miRNAs in C. elegans development (Lee et al. 1993; Wightman et al. 1993; Reinhart et al. 2000) and the very probable roles of miRNAs in plant development (Rhoades et al. 2002), it is tempting to speculate that the substantial expansion of miRNA genes in animals (and the…...
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...This 21-nt RNA pairs to sites within the 3 untranslated region (UTR) of target mRNAs, specifying the translational repression of these mRNAs and triggering the transition to the next developmental stage (Lee et al. 1993; Wightman et al. 1993; Ha et al. 1996; Moss et al. 1997; Olsen and Ambros 1999)....
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...…why in these particular cases, the miRNAs specify translational repression rather than mRNA cleavage during C. elegans larval development (Lee et al. 1993; Wightman et al. 1993; Ha et al. 1996; Moss et al. 1997; Olsen and Ambros 1999; Reinhart et al. 2000; Slack et al. 2000; Hutvágner…...
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...The first such tiny regulatory RNA to be identified was the lin-4 RNA, which controls the timing of Caenorhabditis elegans larval development (Lee et al. 1993; Wightman et al. 1993)....
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9,629 citations
"The microRNAs of Caenorhabditis ele..." refers background in this paper
...…identification of ncRNA genes through comparative genomics, and complex algorithms have been developed to identify ncRNAs in general (Argaman et al. 2001; Rivas et al. 2001; Wassarman et al. 2001), as well as specific ncRNA families such as tRNAs and snoRNAs (Lowe and Eddy 1997, 1999)....
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...…methods to identify ncRNA genes in bacteria (Argaman et al. 2001; Rivas et al. 2001; Wassarman et al. 2001), but is lower than that of algorithms designed to identify protein-coding genes or specialized programs that predict tRNAs and snoRNAs (Lowe and Eddy 1997, 1999; Burge and Karlin 1998)....
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5,229 citations
"The microRNAs of Caenorhabditis ele..." refers background in this paper
...…doublestranded RNA (either a bimolecular duplex or an extended hairpin) is processed by Dicer, an RNAse III enzyme, into many siRNAs that serve as guide RNAs to specify the destruction of the corresponding mRNA (Hammond et al. 2000; Zamore et al. 2000; Bernstein et al. 2001; Elbashir et al. 2001a)....
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...During RNAi, long doublestranded RNA (either a bimolecular duplex or an extended hairpin) is processed by Dicer, an RNAse III enzyme, into many siRNAs that serve as guide RNAs to specify the destruction of the corresponding mRNA (Hammond et al. 2000; Zamore et al. 2000; Bernstein et al. 2001; Elbashir et al. 2001a)....
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