Journal ArticleDOI
The molecular organization of nerve membranes: I. Isolation and characterization of plasma membranes from the retinal axons of the squid: an axolemma-rich preparation
Siegmund Fischer,Marla Cellino,Fernando Zambrano,Guido Zampighi,M. Tellez Nagel,Dita Marcus,Mitzy Canessa-Fischer +6 more
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TLDR
Assays of mitochondrial and endoplasmic reticulum enzymes indicated that rare contamination of these structures was present in F-100 and indicate that two types of plasma membranes chemically and structurally different were purified from the retinal axons of the squid.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-05-01. It has received 73 citations till now. The article focuses on the topics: Membrane & Differential centrifugation.read more
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Journal ArticleDOI
Isolation of plasma membranes from rat brain
TL;DR: Putative neuronal membrane markers, gangliosides and sodium, potassium-dependent ATPase, were enriched over 10-fold in these membranes in comparison to the homogenate, which suggests some contamination with glial cell plasma membrane.
Book ChapterDOI
Role of Cholesterol in Biomembranes and Related Systems
TL;DR: The chapter describes the evidence concerning possible interaction of cholesterol with phospholipids in the aqueous phase from the use of physical techniques and how these subtle interlipid interactions modify the gross functions of membranes.
Journal ArticleDOI
Interaction of the Acetylcholine (Nicotinic) Receptor Protein from Torpedo marmorata Electric Organ with Monolayers of Pure Lipids
TL;DR: Membrane fragments rich in cholinergic (nicotinic) receptor protein were purified from the electric organ of Torpedo marmorata and incorporation experiments into films formed from various pure lipids showed that the protein interacts more readily with cholesterol than with ergosterol, phosphatidylcholine, or other phospholipids.
Journal ArticleDOI
Axons: isolation from mammalian central nervous system.
TL;DR: Centrifugation of a homogenate of white matter, in a solution of buffered sucrose containing salt, produces a floating layer of myelinated axons, although no axolemma or neurotubules are evident.
Journal ArticleDOI
Constitution and properties of axonal membranes of crustacean nerves
TL;DR: The purification of axonal membranes of crustaceans was followed by measuring enrichment in [3H]tetrodotoxin binding capacity and in Na+, K+-ATPase activity and the presence of a nicotine binding component in the axonal membrane of the lobster was confirmed but it was unable to find [ 3H]nicotine binding to crab axonal lipid membranes.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
Quantitative analysis of phospholipids by thin-layer chromatography.
Journal ArticleDOI
Studies on plasma membranes. i. chemical composition and enzyme content of plasma membranes isolated from rat liver
TL;DR: The possibility that the plasma-membrane preparations were contaminated by microsomal elements is discussed, and, on account of the available evidence, the conclusion is reached that there is no reason to assume that this was the case.
Journal ArticleDOI
Qualitative and quantitative colorimetric determination of heptoses.
TL;DR: All heptoses produce with orcinol in dilute HCl two characteristic colored compounds which differ greatly in their absorption spectra, and three different modifications of this reaction were found useful.