Journal ArticleDOI
The mouse excisional wound splinting model, including applications for stem cell transplantation
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TLDR
This protocol describes how to implant stem cells onto the wound bed in Matrigel and/or into the surrounding tissue through injection and how to monitor the engraftment and the effects of stem cells in angiogenesis and wound healing.Abstract:
The mouse excisional wound healing model has been used extensively to study wound healing and cutaneous regeneration. However, as mouse skin is mobile, contraction accounts for a large part of wound closure. In the mouse excisional wound splinting model, a splinting ring tightly adheres to the skin around the wound, preventing local skin contraction. The wound therefore heals through granulation and re-epithelialization, a process similar to that occurring in humans. The model, which takes 2-4 weeks to carry out, can be used to study the effects of stem cells on cutaneous repair or regeneration. In this protocol, we also describe how to implant stem cells onto the wound bed in Matrigel and/or into the surrounding tissue through injection. Serial wound tissue samples at different time points can be harvested to monitor the engraftment and the effects of stem cells in angiogenesis and wound healing.read more
Citations
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Journal ArticleDOI
Epithelialization in Wound Healing: A Comprehensive Review
Irena Pastar,Olivera Stojadinovic,Natalie C. Yin,Horacio Ramirez,Aron G. Nusbaum,Andrew P. Sawaya,Shailee Patel,Laiqua Khalid,R. Isseroff,Marjana Tomic-Canic +9 more
TL;DR: The pivotal role of keratinocytes in epithelialization is focused on, including cellular processes and mechanisms of their regulation during re-epithelialization, and their cross talk with other cell types participating in wound healing.
Journal ArticleDOI
Accelerated wound healing by injectable microporous gel scaffolds assembled from annealed building blocks
TL;DR: An injectable, interconnected microporous gel scaffold assembled from annealed microgel building blocks whose chemical and physical properties can be tailored by microfluidic fabrication facilitated cell migration that resulted in rapid cutaneous-tissue regeneration and tissue-structure formation within five days.
Journal ArticleDOI
Bioinspired mechanically active adhesive dressings to accelerate wound closure.
S. O. Blacklow,Jianyu Li,Benjamin R. Freedman,Benjamin R. Freedman,Mahdi Zeidi,Chao Chen,Chao Chen,David J. Mooney,David J. Mooney +8 more
TL;DR: Novel active adhesive dressings consisting of thermoresponsive tough adhesive hydrogels that combine high stretchability, toughness, tissue adhesion, and antimicrobial function are presented to accelerate wound healing and may find broad utility in applications ranging from regenerative medicine to soft robotics.
Journal ArticleDOI
Two-Pronged Strategy of Biomechanically Active and Biochemically Multifunctional Hydrogel Wound Dressing To Accelerate Wound Closure and Wound Healing
TL;DR: A two-pronged strategy of biomechanically active and biochemically functional hydrogel wound dressing which can assist wound closure and have multiple functions to promote wound healing has been proposed.
Journal ArticleDOI
Wound dressings functionalized with silver nanoparticles: promises and pitfalls
Katayoon Kalantari,Ebrahim Mostafavi,Amalina M. Afifi,Zahra Izadiyan,Hossein Jahangirian,Roshanak Rafiee-Moghaddam,Thomas J. Webster +6 more
TL;DR: This review highlights the therapeutic approaches of using wound dressings functionalized with silver nanoparticles and their potential role in revolutionizing wound healing and the physiology of the skin and wounds is discussed to place the use of Ag-NPs in wound care into perspective.
References
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Journal ArticleDOI
Cutaneous wound healing.
TL;DR: The primary goals of the treatment of wounds are rapid wound closure and a functional and aesthetically satisfactory scar.
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Wound Healing--Aiming for Perfect Skin Regeneration
TL;DR: Details of how these signals control wound cell activities are beginning to emerge, and studies of healing in embryos have begun to show how the normal adult repair process might be readjusted to make it less like patching up and more like regeneration.
Journal ArticleDOI
Wound repair and regeneration
TL;DR: Knowing how organisms retain the ability to regenerate tissue throughout adult life might help to unlock latent regenerative pathways in humans, which would change medical practice as much as the introduction of antibiotics did in the twentieth century.
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Wound healing and its impairment in the diabetic foot
Vincent Falanga,Vincent Falanga +1 more
TL;DR: Enhanced understanding and correction of pathogenic factors, combined with stricter adherence to standards of care and with technological breakthroughs in biological agents, is giving new hope to the problem of impaired healing.
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Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis.
TL;DR: The data suggest that bone marrow‐derived mesenchymal stem cells promote wound healing through differentiation and release of proangiogenic factors.