The nature and dynamics of spermatogonial stem cells
Reads0
Chats0
TLDR
This Review provides an overview of the organization and timing of spermatogenesis and then discusses these models in light of recent studies of SSC markers, heterogeneity and cell division dynamics, highlighting the evidence for and against each model.Abstract:
Spermatogonial stem cells (SSCs) are crucial for maintaining spermatogenesis throughout life, and understanding how these cells function has important implications for understanding male infertility. Recently, various populations of cells harbouring stem cell-like properties have been identified in rodent seminiferous tubules, but deciphering how these cells might fuel spermatogenesis has been difficult, and various models to explain SSC dynamics have been put forward. This Review provides an overview of the organization and timing of spermatogenesis and then discusses these models in light of recent studies of SSC markers, heterogeneity and cell division dynamics, highlighting the evidence for and against each model.read more
Citations
More filters
Journal ArticleDOI
A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq.
Christopher D. Green,Qianyi Ma,Gabriel L. Manske,Adrienne Niederriter Shami,Xianing Zheng,Simone Marini,Lindsay Moritz,Caleb Sultan,Stephen J. Gurczynski,Bethany B. Moore,Michelle D. Tallquist,Jun Li,Saher Sue Hammoud +12 more
TL;DR: High-resolution single-cell RNA sequencing data was collected from ∼35,000 cells from the adult mouse testis and identified all known germ and somatic cells, as well as two unexpected somatic cell types, representing a community resource and foundation of knowledge to study germ cell development and in vivo gametogenesis.
Journal ArticleDOI
Spermatogonial stem cells and spermatogenesis in mice, monkeys and men.
Adetunji P. Fayomi,Kyle E. Orwig +1 more
TL;DR: An overview of stem, progenitor and differentiating sperMatogonia that support spermatogenesis is provided; identifying parallels between rodents and primates where they exist as well as features unique to higher primates.
Journal ArticleDOI
The Neonatal and Adult Human Testis Defined at the Single-Cell Level
Abhishek Sohni,Kun Tan,Hye-Won Song,Dana A. Burow,Dirk G. de Rooij,Louise C. Laurent,Tung-Chin Hsieh,Raja Rabah,Saher Sue Hammoud,Elena Vicini,Miles F. Wilkinson +10 more
TL;DR: Single-cell RNA sequencing to analyze human testes provides a blueprint of the developing human male germline and supporting somatic cells and the PGC-like and SSC markers are candidates to be used for SSC therapy to treat infertility.
Journal ArticleDOI
Spermatogonial stem cells: updates from specification to clinical relevance.
TL;DR: It is suggested that spermatogonia are adapted to the individual reproductive lifespan and that the life-long sperm output from a sperMatogonium is balanced against the duration of a generation, and the risk of jeopardizing genome integrity is balancedAgainst a maximized sperm output.
Journal ArticleDOI
Molecular regulation of spermatogonial stem cell renewal and differentiation.
TL;DR: The SSC niche needs to be understood as a dynamic system that is able to serve the long-term reproductive function and maintenance of fertility both under steady-state and during development plus regeneration.
References
More filters
Journal Article
The relationship between the spleen colony-forming cell and the haemopoietic stem cell
TL;DR: Several experimental findings that are inconsistent with the view that the spleen colony-forming cell (CFU-S) is the primary haemopoietic stem cell are reviewed and a hypothesis is proposed in which the stem cell is seen in association with other cells which determine its behaviour.
Journal ArticleDOI
Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells
Hugo J. Snippert,Laurens G. van der Flier,Toshiro Sato,Johan H. van Es,Maaike van den Born,Carla Kroon-Veenboer,Nick Barker,Allon M. Klein,Jacco van Rheenen,Benjamin D. Simons,Hans Clevers +10 more
TL;DR: Quantitative analysis shows that stem cell turnover follows a pattern of neutral drift dynamics, consistent with a model in which the resident stem cells double their numbers each day and stochastically adopt stem or TA fates.
Journal ArticleDOI
Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
Mito Kanatsu-Shinohara,Narumi Ogonuki,Kimiko Inoue,Hiromi Miki,Atsuo Ogura,Shinya Toyokuni,Takashi Shinohara +6 more
TL;DR: In vitro culture of spermatogonial stem cells that proliferate for long periods of time are reported, and gonocytes isolated from neonatal mouse testis proliferated over a 5-month period and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules.