The new frontier of genome engineering with CRISPR-Cas9
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"The new frontier of genome engineer..." refers background or methods in this paper
...Targeting with multiple sgRNAs—referred to as multiplexing—was also successfully achieved (75, 86)....
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...2013 Cas9-RNA mediates site-specific genome engineering in human cells, other eukaryotes (75, 85, 86) CRISPR biology...
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...1016/0092-8674 (86)90463-0; pmid: 3002636...
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...thermophilus Cas9 (75) were coexpressed with custom-designed sgRNAs (75, 85, 86) or with tracrRNA coexpressed with custom-designed crRNAs (75) in human embryonic kidney, chronic myelogenous leukemia, or induced pluripotent stem cells (75, 85, 86) as well as in mouse cells (75)....
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...(64), three studies in January 2013 demonstrated that CRISPR-Cas9 represents an efficient tool to edit the genomes of human cells (75, 85, 86)....
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"The new frontier of genome engineer..." refers background in this paper
...thermophilus Cas9 is essential for defense against viral invasion (45, 66), might be responsible for introducing DSBs into invading plasmids and phages (67), enables in vivo targeting of temperate phages and plasmids in bacteria (66, 68), and requires the HNH and RuvC domains to interfere with plasmid transformation efficiency (68)....
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...provided the first experimental evidence of CRISPRCas–mediated adaptive immunity (45)....
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...2010 onward Increasing use of TALENs for genome engineering 1979 Gene replacement in yeast (1) 1996–2003 Zinc-finger nucleases for genome engineering (28–30) 2007 CRISPR-Cas is bacterial immune system (45) 2012 CRISPR-Cas9 is RNA-guided DNA endonuclease (64)...
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