Journal ArticleDOI
The polypeptides of influenza virus. 3. Identification of the hemagglutinin, neuraminidase and nucleocapsid proteins.
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TLDR
The hemagglutinin and nucleocapsid protein of the A Bel strain of influenza virus, and the neuraminidase of the B Lee strain have been isolated and analysed by polyacrylamide gel electrophoresis in the presence of neutral sodium dodecyl sulfate.About:
This article is published in Virology.The article was published on 1970-11-01. It has received 81 citations till now. The article focuses on the topics: Hemagglutinin-neuraminidase & Neuraminidase.read more
Citations
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Journal ArticleDOI
Role of hemagglutinin cleavage for the pathogenicity of influenza virus.
TL;DR: F influenza virus pathogenicity is discussed, with an emphasis on the role of HA0 cleavage as a determining factor.
Journal ArticleDOI
The structure of influenza virus. I. The polypeptides of the virion.
TL;DR: Seven polypeptides, varying in size from 24,300 to 92,000 daltons, have been obtained from the WSN strain of influenza virus, and two of them are resistant to degradative enzymes but can be made susceptible to them by treating the particle with phospholipase C.
Book ChapterDOI
Genes and Proteins of the Influenza Viruses
TL;DR: The structure of the influenza virus hemagglutinin complexed with its receptor sialic acid has been elucidated, which may provide a basis for the rational design of antiviral drugs that would block viral attachment to cells.
Journal ArticleDOI
Conserved and host-specific features of influenza virion architecture
Edward C. Hutchinson,Philip D. Charles,Svenja Hester,Benjamin Thomas,David C. Trudgian,Mónica Martínez-Alonso,Ervin Fodor +6 more
TL;DR: This work identifies and quantifies virion proteins with mass spectrometry, producing a complete and quantified model of the hundreds of viral and host-encoded proteins that make up the pleomorphic virions of influenza viruses.
Journal ArticleDOI
The budding mechanisms of enveloped animal viruses.
Kai Simons,Henrik Garoff +1 more
TL;DR: The mechanism of budding bears not only on virus assembly but also perhaps on the assembly of differentiated membrane vesicles in the animal cell (Franke & Kartenbeck, 1976).
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
TL;DR: Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.
Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
Amino Acid Metabolism in Mammalian Cell Cultures
TL;DR: The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in either monolayer or suspension.