The Potential Effect of Fucoidan on Inhibiting Epithelial-to-Mesenchymal Transition, Proliferation, and Increase in Apoptosis for Endometriosis Treatment: In Vivo and In Vitro Study.
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TLDR
For the first time, fucoidan indicated anti-proliferative and anti-inflammatory effects as well as inhibited EMT progression and induced apoptosis, improving endometriosis.Abstract:
Endometriosis is common in reproductive-age women and its pathology is to increase proliferation and migration to enhance epithelial-to-mesenchymal transition progression (EMT). However, treatments are currently limited, so it is important to explore new therapeutic drugs. Hence, in this study, we investigate the therapeutic effect of fucoidan (FC) on the progression and mechanisms of endometriosis. The cell viability of endometrial cell lines End1/E6E7 and Vk2/E6E7 treated with different concentrations of FC were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell counting. Cell migration was evaluated using wound-healing assay. In an in vivo experiment, female Balb/c mice received surgically induced endometriosis followed by different concentrations of fucoidan for 6 weeks. High-frequency ultrasound imaging was applied to detect subsequent lesion growth. The results demonstrated that fucoidan inhibited the viability and migration ability of End1/E6E7 and Vk2/E6E7 cells. Additionally, the administration of fucoidan reduced the volume and weight of endometriotic lesions, decreased inflammatory cytokines and vascular endothelial growth factor (VEGF) of serum and lesions, and improved EMT proliferation and apoptosis-related protein expression. For the first time, fucoidan indicated anti-proliferative and anti-inflammatory effects as well as inhibited EMT progression and induced apoptosis, improving endometriosis.read more
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Pathogenesis of Endometriosis: New Insights into Prospective Therapies.
TL;DR: In this paper, a review of pharmacological inhibitors that can be therapeutically investigated in terms of their effects on signaling pathways and/or mechanisms involved in the pathogenesis of endometriosis is presented.
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Endometriosis in the Mouse: Challenges and Progress Toward a ‘Best Fit’ Murine Model
Katherine A. Burns,Amelia M. Pearson,Jessica L. Slack,Elaine D. Por,Alicia N. Scribner,Nazmin Eti,Richard E. Burney +6 more
TL;DR: A search of the peer-reviewed literature was conducted to identify publications describing preclinical research using a murine model of endometriosis and found three models that offer significant advantages in lesion development and readout toward a high-fidelity mouse model for translational research in endometRIosis.
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Emerging Drug Targets for Endometriosis
TL;DR: In this paper , the authors evaluated endometriosis-related pathways and identified novel therapies to treat it, focusing on the crucial role of inflammation and inflammatory molecules in order to define new perspectives for non-hormonal treatment of the disease by targeting inflammation, nuclear factor kappa B and cytokines, or reactive oxygen species, apoptotic and autophagic pathways, regulators of epithelial-mesenchymal transition, and angiogenesis and neuroangiogenesis.
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SIRT1 upregulation promotes epithelial-mesenchymal transition by inducing senescence escape in endometriosis
TL;DR: In this article , the authors used integrated systems biology analysis and found that enrichment of endometrial stromal fibroblasts in endometriosis and their cellular abundance correlated negatively with epithelial cells in clinical specimens.
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Using pure Fucoidan and radiolabeled Fucoidan (99mTc-Fucoidan) as a new agent for inflammation diagnosis and therapy
TL;DR: In this article , the applicability of Fucoidan as a therapeutic and imaging agent was evaluated in two inflammation models for therapeutic purposes: arthritis and lungs (LPS), and the results demonstrated that Fucoidon has a therapeutic anti-inflammatory effect, especially in the lung model (lung model), and also the imaging application demonstrated that radiolabeledFucoidan has an important chemoattraction for inflammation sites with very high bioaccumulation, which permits to think in an imaging application.
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