The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes
01 Jan 1963-Archives of Biochemistry and Biophysics (Arch Biochem Biophys)-Vol. 100, Iss: 1, pp 119-130
TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.
About: This article is published in Archives of Biochemistry and Biophysics.The article was published on 1963-01-01. It has received 4227 citations till now. The article focuses on the topics: Hemoglobin binding & Spectrin binding.
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8,245 citations
TL;DR: The partition of proteins during phase separation in solutions of Triton X-114 is investigated and integral membrane proteins with an amphiphilic nature are recovered in the detergent phase.
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TL;DR: The lipid composition of the vesicles shows the high sphingomyelin content characteristic of sheep red cell plasma membranes, but not white cell or platelet membranes, consistent with the conclusion that the vESicles are of reticulocyte origin.
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TL;DR: A top-down biomimetic approach in particle functionalization is reported by coating biodegradable polymeric nanoparticles with natural erythrocyte membranes, including both membrane lipids and associated membrane proteins for long-circulating cargo delivery.
Abstract: Efforts to extend nanoparticle residence time in vivo have inspired many strategies in particle surface modifications to bypass macrophage uptake and systemic clearance. Here we report a top-down biomimetic approach in particle functionalization by coating biodegradable polymeric nanoparticles with natural erythrocyte membranes, including both membrane lipids and associated membrane proteins for long-circulating cargo delivery. The structure, size and surface zeta potential, and protein contents of the erythrocyte membrane-coated nanoparticles were verified using transmission electron microscopy, dynamic light scattering, and gel electrophoresis, respectively. Mice injections with fluorophore-loaded nanoparticles revealed superior circulation half-life by the erythrocyte-mimicking nanoparticles as compared to control particles coated with the state-of-the-art synthetic stealth materials. Biodistribution study revealed significant particle retention in the blood 72 h following the particle injection. The translocation of natural cellular membranes, their associated proteins, and the corresponding functionalities to the surface of synthetic particles represents a unique approach in nanoparticle functionalization.
1,614 citations
Cites methods from "The preparation and chemical charac..."
...RBC ghosts devoid of cytoplasmic contents were prepared following previously published protocols with modifications (32)....
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TL;DR: The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and 125I-labeled anti-transferrin-receptor antibodies and it can be shown that at 0 degree C or in phosphate-buffered saline the rate of vesicle release is less than that at 37 degrees C in culture medium.
1,543 citations
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TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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