The preparation and properties of two new chromogenic substrates of trypsin.
01 Nov 1961-Archives of Biochemistry and Biophysics (Arch Biochem Biophys)-Vol. 95, Iss: 2, pp 271-278
TL;DR: Preliminary studies indicate that benzoyl dl -arginine p -nitroanilide hydrochloride is also hydrolyzed by papain, and that of l -LPA is in a more alkaline region than normally found for trypsin substrates.
About: This article is published in Archives of Biochemistry and Biophysics.The article was published on 1961-11-01. It has received 3118 citations till now. The article focuses on the topics: Trypsin.
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14 Apr 2006
3,340 citations
TL;DR: In this paper, the Electrophoretic α, 1-Globulin Pattern of Serum in α; 1-Antitrypsin Deficiency; and 1-Girdhar et al.
Abstract: (1963). The Electrophoretic α;1-Globulin Pattern of Serum in α;1-Antitrypsin Deficiency. Scandinavian Journal of Clinical and Laboratory Investigation: Vol. 15, No. 2, pp. 132-140.
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TL;DR: Two hundred forty male Avian Farms broiler chicks, 1 d of age, were randomly allocated to four treatments, each of which had five pens of 12 chicks per pen, to investigate the effects of fructooligosaccharide (FOS) on digestive enzyme activities and intestinal microflora and morphology.
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TL;DR: The synthesis and analytical use of succinyl-( l -alanine) 3 - p -nitroanilide, a new elastase substrate that is virtually not attacked by trypsin or chymotrypsin and does not undergo appreciable spontaneous hydrolysis is described.
709 citations
TL;DR: Two tetrapeptide 4-nitroanilide substrates related to the reactive site of the plasma (~1’ protease inhibitor) were studied and indicate that oxidation of the PI Met of YePI is capable to destroying its reactivity toward most serine proteases.
610 citations
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TL;DR: On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, where (E:l = (ES).
Abstract: On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, (ES), a t all values of the concentration of the substrate, (S). It is proportional to (S) only a t low values of (S). The numerical value of the dissociation constant is given by the substrate concentration a t half-maximum velocity, where (E:l = (ES). The equilibrium in equation 1 may be heterogeneous or homogeneous. Hitchcock'\" has pointed
11,349 citations
1,147 citations
340 citations
TL;DR: In the course of an investigation of the inhibition of crystalline pancreatic proteolytic enzymes by specific low molecular weight compounds the discovery was made that crystalline trypsin is likewise a powerful catalyst for the hydrolysis of certain amino acid esters.
308 citations
258 citations