The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose.
TL;DR: Thy-1mutant mouse lymphoma cells have the a1,3-mannosyltransferase necessary for the conversion of the Man5GlCNAcz species to Man,GlcNAcz, but lack the appropriate mannosy1 donor (dolichol-P-mannose)necessary for the formation of the larger lipid-linked oligosaccharides.
About: This article is published in Journal of Biological Chemistry.The article was published on 1980-05-25 and is currently open access. It has received 211 citations till now. The article focuses on the topics: Mannose & Glycosylation.
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TL;DR: A number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport, such as tunicamycin, tridecaptin, and flavomycin this paper.
Abstract: A number of glycoproteins have oligosaccharides linked to protein in a GlcNAc----asparagine bond. These oligosaccharides may be either of the complex, the high-mannose or the hybrid structure. Each type of oligosaccharides is initially biosynthesized via lipid-linked oligosaccharides to form a Glc3Man9GlcNAc2-pyrophosphoryl-dolichol and transfer of this oligosaccharide to protein. The oligosaccharide portion is then processed, first of all by removal of all three glucose residues to give a Man9GlcNAc2-protein. This structure may be the immediate precursor to the high-mannose structure or it may be further processed by the removal of a number of mannose residues. Initially four alpha 1,2-linked mannoses are removed to give a Man5 - GlcNAc2 -protein which is then lengthened by the addition of a GlcNAc residue. This new structure, the GlcNAc- Man5 - GlcNAc2 -protein, is the substrate for mannosidase II which removes the alpha 1,3- and alpha 1,6-linked mannoses . Then the other sugars, GlcNAc, galactose, and sialic acid, are added sequentially to give the complex types of glycoproteins. A number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport. Some of these inhibitors have been valuable tools to study the reaction pathways while others have been extremely useful for examining the role of carbohydrate in glycoprotein function. For example, tunicamycin and its analogs prevent protein glycosylation by inhibiting the first step in the lipid-linked pathway, i.e., the formation of Glc NAc-pyrophosphoryl-dolichol. These antibiotics have been widely used in a number of functional studies. Another antibiotic that inhibits the lipid-linked saccharide pathway is amphomycin, which blocks the formation of dolichyl-phosphoryl-mannose. In vitro, this antibiotic gives rise to a Man5GlcNAc2 -pyrophosphoryl-dolichol from GDP-[14C]mannose, indicating that the first five mannose residues come directly from GDP-mannose rather than from dolichyl-phosphoryl-mannose. Other antibodies that have been shown to act at the lipid-level are diumycin , tsushimycin , tridecaptin, and flavomycin. In addition to these types of compounds, a number of sugar analogs such as 2-deoxyglucose, fluoroglucose , glucosamine, etc. have been utilized in some interesting experiments. Several compounds have been shown to inhibit glycoprotein processing. One of these, the alkaloid swainsonine , inhibits mannosidase II that removes alpha-1,3 and alpha-1,6 mannose residues from the GlcNAc- Man5GlcNAc2 -peptide. Thus, in cultured cells or in enveloped viruses, swainsonine causes the formation of a hybrid structure.(ABSTRACT TRUNCATED AT 400 WORDS)
1,067 citations
01 Jan 1987
TL;DR: A number of glycoproteins have oligosaccharides linked to protein in a GlcNAc----asparagine bond that are either of the complex, the high-mannose or the hybrid structure and a number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport.
900 citations
TL;DR: Etude de la synthese des groupements fonctionnels glycosyl-phosphatidylinositol se liant aux extremites C terminales des proteines procaryotique ou eucaryotiques, permettant leur determination et leur identification.
485 citations
TL;DR: A trypanosome cell-free system capable of performing all of the steps in the biosynthesis of the glycan portion of glycolipid A and a proposed pathway for GPI biosynthesis is proposed.
283 citations
TL;DR: This review may familiarize ‘newcomers’ with the field of glycoconjugate research with special emphasis on glycoprotein glycans and current knowledge on functional roles of glycans is presented.
Abstract: Since the pioneering work on structure and function of heteroglycans compiled in the classical books edited by A. Gottschalk in 19721, there have been several promising developments in glycoconjugate research, as reviewed in this article.
262 citations
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TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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TL;DR: It is proposed that during glycosylation of asparagine residues, a common oligosaccharide is transferred from the lipid carrier to protein and is subsequently processed to yield the so-called "high mannose" and "complex" oligOSaccharides.
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