Journal ArticleDOI
The second wave of synthetic biology: from modules to systems
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TLDR
To view cells as true 'programmable' entities, it is now essential to develop effective strategies for assembling devices and modules into intricate, customizable larger scale systems.Abstract:
Synthetic biology is a research field that combines the investigative nature of biology with the constructive nature of engineering. Efforts in synthetic biology have largely focused on the creation and perfection of genetic devices and small modules that are constructed from these devices. But to view cells as true 'programmable' entities, it is now essential to develop effective strategies for assembling devices and modules into intricate, customizable larger scale systems. The ability to create such systems will result in innovative approaches to a wide range of applications, such as bioremediation, sustainable energy production and biomedical therapies.read more
Citations
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Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Dynamic DNA nanotechnology using strand-displacement reactions
David K.Y. Zhang,Georg Seelig +1 more
TL;DR: Here, this work reviews DNA strand-displacement-based devices, and looks at how this relatively simple mechanism can lead to a surprising diversity of dynamic behaviour.
Journal ArticleDOI
Genetic circuit design automation
Alec A. K. Nielsen,Bryan S. Der,Bryan S. Der,Jonghyeon Shin,Prashant Vaidyanathan,Vanya Paralanov,Elizabeth A. Strychalski,David J. Ross,Douglas Densmore,Christopher A. Voigt +9 more
TL;DR: Electronic design automation principles from EDA are applied to enable increased circuit complexity and to simplify the incorporation of synthetic gene regulation into genetic engineering projects, and it is demonstrated that engineering principles can be applied to identify and suppress errors that complicate the compositions of larger systems.
PatentDOI
Orthogonal cas9 proteins for rna-guided gene regulation and editing
TL;DR: In this article, the use of multiple orthogonal Cas9 proteins to simultaneously regulate corresponding genes or simultaneously and independently edit corresponding genes is described. But the authors do not consider the effect of the Cas9 protein on the expression of the target nucleic acid.
Journal ArticleDOI
Precise and reliable gene expression via standard transcription and translation initiation elements
Vivek K. Mutalik,Joao C. Guimaraes,Joao C. Guimaraes,Guillaume Cambray,Colin Lam,Marc Juul Christoffersen,Quynh-Anh Mai,Andrew B Tran,Morgan L Paull,Jay D. Keasling,Adam P. Arkin,Adam P. Arkin,Drew Endy +12 more
TL;DR: An expression cassette architecture for genetic elements controlling transcription and translation initiation in Escherichia coli is developed, demonstrating that arbitrary genes are reliably expressed to within twofold relative target expression windows with ∼93% reliability.
References
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Journal ArticleDOI
Stochastic Gene Expression in a Single Cell
TL;DR: This work constructed strains of Escherichia coli that enable detection of noise and discrimination between the two mechanisms by which it is generated and reveals how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation.
Book
Stability and Complexity in Model Ecosystems
Robert M. May,N. MacDonald +1 more
TL;DR: Preface vii Preface to the Second Edition Biology Edition 1.
Journal ArticleDOI
A synthetic oscillatory network of transcriptional regulators
TL;DR: This work used three transcriptional repressor systems that are not part of any natural biological clock to build an oscillating network, termed the repressilator, in Escherichia coli, which periodically induces the synthesis of green fluorescent protein as a readout of its state in individual cells.
Journal ArticleDOI
Construction of a genetic toggle switch in Escherichia coli
TL;DR: The construction of a genetic toggle switch is presented—a synthetic, bistable gene-regulatory network—in Escherichia coli and a simple theory is provided that predicts the conditions necessary for bistability.