The Shielding Action of Disaccharides for Typical Proteins in Aqueous Solution Against Static, 50 Hz and 1800 MHz Frequencies Electromagnetic Fields
About: This article is published in Current Chemical Biology.The article was published on 2016-03-31. It has received 20 citations till now. The article focuses on the topics: Electromagnetic shielding.
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TL;DR: In this paper, IR spectra on water mixtures of two homologous disaccharides, i.e., sucrose and trehalose, as a function of temperature have been collected.
Abstract:
In the present paper, InfraRed (IR) spectra on water mixtures of two homologous disaccharides, i.e. sucrose and trehalose, as a function of temperature have been collected.
In particular, IR spectra were registered, in the spectral range from 4000 cm-1 to 400 cm-1, to investigate the thermal response of the water mixtures of two homologous disaccharides, through positive thermal scans, i.e. by increasing the temperature from the value of 25°C to the value of 50°C. The OH-stretching region has been analyzed by means of two simple and straightforward procedures, i.e. by evaluating the shift of the intramolecular OH stretching center frequency and the Spectral Distance (SD).
Both the analyses indicate that trehalose water mixture have a higher thermal response than that of the sucrose-water mixture.
20 citations
TL;DR: In this article, a typical tetrameric protein, the hemoglobin, at the concentration of 150 mg/ml in bidistilled water solution, were exposed to a uniform magnetic field at 200 mT at different temperatures of 15 ∘ C, 40 ∘C and 65 ∘ c.
18 citations
01 Jan 2018
TL;DR: In this article, the authors applied laser techniques to acoustically levitated droplets of trehalose aqueous solutions in order to perform spectroscopic analyses as a function of concentration and to test the theoretical diameter law.
Abstract: This work reports the results of an experimental study where laser techniques are applied to acoustically levitated droplets of trehalose aqueous solutions in order to perform spectroscopic analyses as a function of concentration and to test the theoretical diameter law. The study of such systems is important in order to better understand the behaviour of trehalose-synthesizing extremophiles that live in extreme environments. In particular, it will be shown how acoustic levitation, combined with optical spectroscopic instruments allows to explore a wide concentration range and to test the validity of the diameter law as a function of levitation lag time, i.e. the D2 vs t law. On this purpose a direct diameter monitoring by a video camera and a laser pointer was first performed; then the diameter was also evaluated by an indirect measure through an OH/CH band area ratio analysis of collected Raman and Infrared spectra. It clearly emerges that D2 vs t follows a linear trend for about 20 minutes, reaching then a plateau at longer time. This result shows how trehalose is able to avoid total water evaporation, this property being essential for the surviving of organisms under extreme environmental conditions.
11 citations
TL;DR: These findings can be easily explained assuming that proteins α-helices and lipids contents in cellular membrane aligned toward the direction of the applied magnetic field after exposure, inducing an increase of ions flux across cellular membrane channels after exposure to a magnetic field, changing cellular functions.
Abstract: Purpose: To study the response of neuronal-like cells to an applied static or low-frequency magnetic field.Materials and methods: Fourier Transform Infrared (FTIR) Spectroscopy was used to investig...
11 citations
TL;DR: In this paper, the shielding action of sodium chloride in bidistilled water solution against exposure to a high frequency electromagnetic field, in order to evaluate if the addition of NaCl in proteins aqueous solution can be considered a valuable bioprotector against electromagnetic fields.
Abstract: Background: Previous studies have shown that exposure to high frequency electromagnetic fields induces alterations in simple organic systems such as proteins in bidistilled water solution. Objective: The aim of this study was to test the shielding action of sodium chloride in bidistilled water solution against exposure to a high frequency electromagnetic field, in order to evaluate if the addition of NaCl in proteins aqueous solution can be considered a valuable bioprotector against electromagnetic fields. Method: Samples of 250 μl of different hemoglobin aqueous solutions, in the absence or presence of sodium-chloride, were exposed for 3 hours to an electromagnetic field at 1750 MHz at a power density around 1 W/m 2 emitted by an operational mobile phone. Fourier Transform Infrared Spectroscopy was used to study the effects of exposure on the secondary structure of hemoglobin also in the presence of sodium-chloride. Results: Spectral analysis evidenced that significant increase in intensity of the Amide I and II vibration bands in hemoglobin bidistilled water solution occurred after exposure to the electromagnetic field. This result can be due to the increase of dipole moment of the protein due to the alignment of α-helix towards the direction of the field. In contrast, no appreciable change was observed in hemoglobin in sodium-chloride water solution after exposure. This protective effect of sodium-chloride can be explained by the orientation of water molecules due to the strong electric field around each ion that reduces the possibility of rotation of the protein in response to an applied electromagnetic field.
9 citations
Cites background or result from "The Shielding Action of Disaccharid..."
...This delicate equilibrium can be changed by exposure to HF-EMFs, such as it was shown in previous research [12, 14, 16, 17]....
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...Following these results, the effects of the use of disaccharides as bioprotectors against EMFs were investigated [17, 19 - 21]....
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