The Spliceosome: Design Principles of a Dynamic RNP Machine
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Cites background from "The Spliceosome: Design Principles ..."
...…proteins to form the spliceosome, which undergoes a dynamic series of ordered (and reversible) conformational alterations and protein exchanges (Hoskins and Moore, 2012; Wahl et al., 2009), leading to the first and second steps of splicing and ultimate release of the lariat intron and spliced RNA....
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...The snRNPs assemble on an intron along with a host of proteins to form the spliceosome, which undergoes a dynamic series of ordered (and reversible) conformational alterations and protein exchanges (Hoskins and Moore, 2012; Wahl et al., 2009), leading to the first and second steps of splicing and ultimate release of the lariat intron and spliced RNA....
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1,746 citations
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Cites background from "The Spliceosome: Design Principles ..."
...As the U6 snRNA appears to have lost most of its preactivation binding partners, it not only engages in novel base pairing interactions with U2 but also new protein–RNA interactions are thought to be established (Wahl et al. 2009)....
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...Cite as Cold Spring Harb Perspect Biol 2011;3:a003707 9 are loosely associated and/or have redundant functions (e.g., different SR-proteins) and thus are likely not required to splice every pre-mRNA substrate (Wahl et al. 2009)....
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...pairing interactions with U2 but also new protein–RNA interactions are thought to be established (Wahl et al. 2009)....
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...A dramatic exchange of proteins occurs during spliceosome assembly and activation (Wahl et al. 2009)....
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..., different SR-proteins) and thus are likely not required to splice every pre-mRNA substrate (Wahl et al. 2009)....
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References
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"The Spliceosome: Design Principles ..." refers background in this paper
...…a large number o proteins that are either natively disordered (intrinsically unstruc tured proteins, IUPs) or bear sizeable regions that lack stable tertiary structure (intrinsically unstructured regions, IURs) do not follow this structure-function paradigm (reviewed in Dyson and Wright, 2005)....
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2,590 citations
"The Spliceosome: Design Principles ..." refers background in this paper
...The SR protein ASF/SF2 and hnRNP A1 are a well-characterized example of factors that exhibit antagonistic effects (Black, 2003; Caceres et al., 1994; Zhu et al., 2001)....
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...…spliced, that is, they are present in every mRNA produced from a given pre-mRNA, many are alternatively spliced (especially in higher eukaryotes) to generate variable forms of mRNA from a single pre-mRNA species (reviewed in Black, 2003; Blencowe, 2006; Graveley, 2001; Smith and Valcarcel, 2000)....
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2,218 citations
"The Spliceosome: Design Principles ..." refers background in this paper
...Recognition and selection of splice sites is in most cases influenced by flanking pre-mRNA regulatory sequences—so-called intronic and exonic splicing enhancers or silencers—that can have positive or negative effects on splice-site usage (Cartegni et al., 2002; Singh and Valcarcel, 2005)....
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1,436 citations
"The Spliceosome: Design Principles ..." refers background in this paper
..., 2002), have led to the hypothesis that the spliceosome can also exist in a more extensively preassembled form selection, and glycerol gradient centrifugation), landmark assembly intermediates are operationally defined by the sequential association and release of the spliceosomal snRNPs (reviewed in Brow, 2002; Will and Lührmann, 2006)....
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...…the spliceosome can also exist in a more extensively preassembled form selection, and glycerol gradient centrifugation), landmark assembly intermediates are operationally defined by the sequential association and release of the spliceosomal snRNPs (reviewed in Brow, 2002; Will and Lührmann, 2006)....
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