The TetR Family of Regulators
Reads0
Chats0
TLDR
The most important open question concerning TFR biology is the nature and diversity of their ligands and how these relate to the biochemical processes under their control.Abstract:
SUMMARY The most common prokaryotic signal transduction mechanisms are the one-component systems in which a single polypeptide contains both a sensory domain and a DNA-binding domain. Among the >20 classes of one-component systems, the TetR family of regulators (TFRs) are widely associated with antibiotic resistance and the regulation of genes encoding small-molecule exporters. However, TFRs play a much broader role, controlling genes involved in metabolism, antibiotic production, quorum sensing, and many other aspects of prokaryotic physiology. There are several well-established model systems for understanding these important proteins, and structural studies have begun to unveil the mechanisms by which they bind DNA and recognize small-molecule ligands. The sequences for more than 200,000 TFRs are available in the public databases, and genomics studies are identifying their target genes. Three-dimensional structures have been solved for close to 200 TFRs. Comparison of these structures reveals a common overall architecture of nine conserved α helices. The most important open question concerning TFR biology is the nature and diversity of their ligands and how these relate to the biochemical processes under their control.read more
Citations
More filters
Journal ArticleDOI
The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria
TL;DR: This article highlights the recent progress obtained for organisms of clinical significance, together with methodological considerations for the characterization of MDR pumps, with particular focus on AcrAB-TolC and Mex pumps.
Journal ArticleDOI
Stress Physiology of Lactic Acid Bacteria
Konstantinos Papadimitriou,Ángel Alegría,Peter A. Bron,Maria De Angelis,Marco Gobbetti,Michiel Kleerebezem,José A. Lemos,Daniel M. Linares,Paul Ross,Catherine Stanton,Francesca Turroni,Douwe van Sinderen,Pekka Varmanen,Marco Ventura,Manuel Zúñiga,Effie Tsakalidou,Jan Kok +16 more
TL;DR: The state of the art for LAB stress behavior is presented, and the stress defense mechanisms that have been reported to date are concentrated on, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope.
Journal ArticleDOI
“Deadman” and “Passcode” microbial kill switches for bacterial containment
TL;DR: Two engineered safe-guard systems are presented: the Deadman and Passcode kill switches, which efficiently kill Escherichia coli and can be readily reprogrammed to change their environmental inputs, regulatory architecture and killing mechanism.
Journal ArticleDOI
Cell-free biosensors for rapid detection of water contaminants
Jaeyoung K. Jung,Khalid K. Alam,Matthew S. Verosloff,Daiana A. Capdevila,Morgane Desmau,Phillip R. Clauer,Jeong Wook Lee,Peter Q. Nguyen,Pablo A. Pastén,S. J. Matiasek,Jean François Gaillard,David P. Giedroc,James J. Collins,Julius B. Lucks +13 more
TL;DR: A cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water, and it is shown that adding RNA circuitry can invert responses, reduce crosstalk and improve sensitivity without protein engineering.
Journal ArticleDOI
The Cell Wall Lipid PDIM Contributes to Phagosomal Escape and Host Cell Exit of Mycobacterium tuberculosis.
Jeff Quigley,V. Keith Hughitt,Carlos A. Velikovsky,Roy A. Mariuzza,Najib M. El-Sayed,Volker Briken +5 more
TL;DR: A loss-of-function genetic approach showed that PDIM levels directly correlate with the capacity of M. tuberculosis to escape the phagosome and induce host cell necrosis and macroautophagy, and a novel role of the cell wall lipid PDIM in intracellular host cell modulation, which is important for host cell exit and dissemination of M tuberculosis.
References
More filters
Journal ArticleDOI
Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)
Stephen D. Bentley,Keith F. Chater,Ana Cerdeño-Tárraga,Gregory L. Challis,Gregory L. Challis,Nicholas R. Thomson,Keith D. James,David Harris,Michael A. Quail,H. M. Kieser,D. Harper,Alex Bateman,Steve D.M. Brown,Govind Chandra,Carton W. Chen,Mark O. Collins,Ann Cronin,Andrew G. Fraser,Arlette Goble,J. Hidalgo,T. Hornsby,S. Howarth,Chih-Hung Huang,Tobias Kieser,L. Larke,Lee Murphy,Karen Oliver,Susan O'Neil,Ester Rabbinowitsch,Marie-Adèle Rajandream,Kim Rutherford,Simon Rutter,Kathy Seeger,David L. Saunders,Sarah Sharp,R. Squares,S. Squares,K. Taylor,T. Warren,Andreas Wietzorrek,John Woodward,Bart Barrell,Julian Parkhill,David A. Hopwood +43 more
TL;DR: The 8,667,507 base pair linear chromosome of Streptomyces coelicolor is reported, containing the largest number of genes so far discovered in a bacterium.
Journal ArticleDOI
Sampling the Antibiotic Resistome
TL;DR: This work has shown that soil-dwelling bacteria are a reservoir of resistance determinants that can be mobilized into the microbial community, and study of this reservoir could provide an early warning system for future clinically relevant antibiotic resistance mechanisms.
Journal ArticleDOI
Efflux-Mediated Drug Resistance in Bacteria
Xian-Zhi Li,Hiroshi Nikaido +1 more
TL;DR: Fluoroquinolones and β-lactams of the latest generations are likely to select for overproduction mutants of these pumps and make the bacteria resistant in one step to practically all classes of antibacterial agents.
Journal ArticleDOI
The TetR Family of Transcriptional Repressors
Juan L. Ramos,Manuel Martínez-Bueno,Antonio J. Molina-Henares,Wilson Terán,Kazuya Watanabe,Xiaodong Zhang,María-Trinidad Gallegos,Richard G. Brennan,Raquel Tobes +8 more
TL;DR: A general profile for the proteins of the TetR family of repressors is developed, made up of 47 amino acid residues that correspond to the helix-turn-helix DNA binding motif and adjacent regions in the three- dimensional structures of TetR, QacR, CprB, and EthR, four family members for which the function and three-dimensional structure are known.
Journal ArticleDOI
The Small RNA Chaperone Hfq and Multiple Small RNAs Control Quorum Sensing in Vibrio harveyi and Vibrio cholerae
Derrick H. Lenz,Kenny C. Mok,Brendan N. Lilley,Rahul V. Kulkarni,Ned S. Wingreen,Bonnie L. Bassler +5 more
TL;DR: It is proposed that Hfq, together with four candidate sRNAs, creates an ultrasensitive regulatory switch that controls the critical transition into the high cell density, quorum-sensing mode.
Related Papers (5)
Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)
Stephen D. Bentley,Keith F. Chater,Ana Cerdeño-Tárraga,Gregory L. Challis,Gregory L. Challis,Nicholas R. Thomson,Keith D. James,David Harris,Michael A. Quail,H. M. Kieser,D. Harper,Alex Bateman,Steve D.M. Brown,Govind Chandra,Carton W. Chen,Mark O. Collins,Ann Cronin,Andrew G. Fraser,Arlette Goble,J. Hidalgo,T. Hornsby,S. Howarth,Chih-Hung Huang,Tobias Kieser,L. Larke,Lee Murphy,Karen Oliver,Susan O'Neil,Ester Rabbinowitsch,Marie-Adèle Rajandream,Kim Rutherford,Simon Rutter,Kathy Seeger,David L. Saunders,Sarah Sharp,R. Squares,S. Squares,K. Taylor,T. Warren,Andreas Wietzorrek,John Woodward,Bart Barrell,Julian Parkhill,David A. Hopwood +43 more