The thiobarbituric acid assay of sialic acids.
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TLDR
This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.About:
This article is published in Journal of Biological Chemistry.The article was published on 1959-08-01 and is currently open access. It has received 6264 citations till now. The article focuses on the topics: Sialic acid & Neuraminic acid.read more
Citations
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Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
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Metabolism of isolated fat cells. i. effects of hormones on glucose metabolism and lipolysis.
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Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury.
TL;DR: The conclusion is reached that MDA determination and the TBA test can offer, at best, a narrow and somewhat empirical window on the complex process of lipid peroxidation.
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Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems.
TL;DR: Alkaline pyridine-butanol mixture dissolves both proteins and lipids that are precipitated or opalescent during the reaction, yielding a more accurate MDA estimation as well as simplifying the procedure.
References
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The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.
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The reaction between thiobarbituric acid and the oxidation products of certain lipides.
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A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of the malonaldehyde-thiobarbituric acid reaction.
V.S. Waravdekar,L.D. Saslaw +1 more
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Quantitative Estimation of Sialic Acids. III. An Anion Exchange Resin Method.
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Thiobarbituric Acid Spray Reagent for Deoxy Sugars and Sialic Acids
TL;DR: An adaptation of these methods for spraying paper chromatograms for detecting the products of periodate oxidation, malonaldehyde from deoxy sugars and β-formylpyruvic acid from the latter two groups of compounds is reported.