The Transcriptional Landscape of the Yeast Genome Defined by RNA Sequencing
Citations
20,335 citations
Cites methods from "The Transcriptional Landscape of th..."
...source tool that has been reported and used in short-read projects [6,28]....
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18,940 citations
14,524 citations
Cites methods from "The Transcriptional Landscape of th..."
...A simple quantification method that was used in some initial RNA-Seq papers [13,14] and that is still used today is to count the number of reads that map uniquely to each gene, possibly correcting a gene’s count by the “mappability” of its sequence [15] and its length....
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13,356 citations
Cites background from "The Transcriptional Landscape of th..."
...[1] performed RNA-Seq on replicates of Saccharomyces cerevisiae cultures....
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...This is confirmed by comparison of technical with biological replicates [1]....
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...A common feature between these assays is that they sequence large amounts of DNA fragments that reflect, for example, a biological system’s repertoire of RNA molecules (RNASeq [1,2]) or the DNA or RNA interaction regions of nucleotide binding molecules (ChIP-Seq [3], HITS-CLIP [4])....
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...However, it has been noted [1,8] that the assumption of Poisson distribution is too restrictive: it predicts smaller variations than what is seen in the data....
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13,337 citations
Additional excerpts
...1621" language="eng" relation="no"> High-throughput mRNA sequencing (RNA-Seq) promises simultaneous transcript discovery and abundance estimatio...
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References
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