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Thin Layer Chromatography
Esso1
01 Jan 1963-
TL;DR: The idea of using a chromatographic adsorbent in the form of a thin layer fixed on an inert rigid support seems to have been suggested by Izmailov and Shraiber in 1938.
Abstract: The idea of using a chromatographic adsorbent in the form of a thin layer fixed on an inert rigid support seems to have been suggested by Izmailov and Shraiber in 1938. Meinhard and Hall[1] in 1949 developed this notion of an ‘open column’, and in 1951 Kirchner, Miller, and Keller[2] reported the separation of terpenes on a ‘chromatostrip’, prepared by coating a small glass strip with an adsorbent mixed with starch or plaster of Paris, which acted as a binder. The strips were handled in the same way that paper is handled in paper chromatography, and indeed the original object of the thin-layer technique was to apply the methods of paper partition chromatography to an adsorption system.
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TL;DR: The classes, functions and the representatives of the frequently detected PPCPs in aquatic environments were summarized and suggestions are made for future study of P PCPs.
997 citations
TL;DR: All the various techniques are reviewed here and, in order to unify the different criteria and parameters, standard methods to study the antimicrobial activity of medicinal plants are proposed.
853 citations
TL;DR: A survey of the phospholipid composition of 97 strains representing 20 genera of the Actinomycetales showed that five groups could be distinguished on the basis of the presence or absence of certain nitrogenous phospholips, with chemical heterogeneity of the genera Actinomadura, Corynebacterium, Micropolyspora and Nocardia.
799 citations
TL;DR: Concentrations of the four glycosyl ceramides in plasma and in erythrocytes were determined for samples from young, healthy males and varied with the complexity of the oligosaccharide moiety and ranged from 94% with glucosyl ceramide to 71% with globoside.
642 citations
TL;DR: The chemical structure of long-chain polyamines (up to 20 repeated units), which represent the main organic constituent of diatom biosilica, are described, which are the longest polyamine chains found in nature and induce rapid silica precipitation from a silicic acid solution.
Abstract: Biomineralizing organisms use organic molecules to generate species-specific mineral patterns. Here, we describe the chemical structure of long-chain polyamines (up to 20 repeated units), which represent the main organic constituent of diatom biosilica. These substances are the longest polyamine chains found in nature and induce rapid silica precipitation from a silicic acid solution. Each diatom is equipped with a species-specific set of polyamines and silica-precipitating proteins, which are termed silaffins. Different morphologies of precipitating silica can be generated by polyamines of different chain lengths as well as by a synergistic action of long-chain polyamines and silaffins.
629 citations
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TL;DR: In this article, the application of thin-layer adsorption chromatography to the fractionation of complex lipid mixtures into classes was discussed, and further work has been done on the use of thin layer chromatography for this purpose.
Abstract: Further work has been done on the application of thin-layer adsorption chromatography to the fractionation of complex lipid mixtures into classes.
367 citations
TL;DR: A novel multidimensional technique has been elaborated and found suitable for the chromatographic identification of 52 amino acids and the relative merits of cellulose and silicagel as carriers are discussed in relation to separation efficiency, reproducibility and sensitivity of detection.
179 citations
TL;DR: Thin-film silicic acid chromatography has been demonstrated to be a useful technique for the separation of a wide variety of partially and completely derivatized carbohydrates.
130 citations
TL;DR: In this article, analytischen und mikropraparativen Schnelltrennung von Stoffgemischen dient in steigendem Mase die Chromatographie in dunnen, feinkornigen Schichten.
Abstract: Zur analytischen und mikropraparativen Schnelltrennung von Stoffgemischen dient in steigendem Mase die Chromatographie in dunnen, feinkornigen Schichten. Auf aktiven Schichten last sich ahnlich wie bei adsorptionschromatographischen Saulentrennungen arbeiten und auf inaktiven Schichten sind verteilungschromatographische Schnelltrennungen moglich. Einfache Handhabung und Schnelligkeit machen die Dunnschicht-Chromatographie in vielen Laboratorien zu einem unentbehrlichen Hilfsmittel.
126 citations
TL;DR: The relation between the molecular weights of proteins and their behaviour on Sephadex gels is discussed and a method for the estimation of protein molecular weights from the results of thin-layer chromatography is derived.
124 citations