Journal ArticleDOI
Thirty years of Escherichia coli DNA gyrase: from in vivo function to single-molecule mechanism.
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TLDR
The biological roles of gyrase in vivo and its mechanism in vitro are reviewed and a unique ability that arises from the specialized C-terminal DNA wrapping domain of the GyrA subunit of type II topoisomerase is described.About:
This article is published in Biochimie.The article was published on 2007-04-01. It has received 123 citations till now. The article focuses on the topics: DNA gyrase & DNA supercoil.read more
Citations
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Journal ArticleDOI
Recent Advances in Optical Tweezers
TL;DR: Although technical in nature, these developments have important implications for the expanded use of optical tweezers in biochemical research and thus should be of general interest.
Journal ArticleDOI
Exploiting bacterial DNA gyrase as a drug target: current state and perspectives.
TL;DR: Known gyrase-specific drugs and toxins are reviewed and the prospects for developing new antibacterials targeted to this enzyme are assessed.
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DNA topoisomerases: harnessing and constraining energy to govern chromosome topology.
TL;DR: How structural and biochemical studies have furthered the understanding of DNA topoisomerases is described, with an emphasis on how these complex molecular machines use interfacial interactions to harness and constrain the energy required to manage DNA topology.
Journal ArticleDOI
Metabolic Control of Persister Formation in Escherichia coli
TL;DR: It is discovered that carbon source transitions stimulate the formation of fluoroquinolone persisters in Escherichia coli and this pathway spans from initial stress to antibiotic target and demonstrates that TA behavior can be exhibited by a metabolite-enzyme interaction (ppGpp-SpoT), in contrast to classical TA systems that involve only protein and/or RNA.
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Interplay in the selection of fluoroquinolone resistance and bacterial fitness.
TL;DR: Data suggest that natural selection for improved growth in bacteria with low-level resistance to fluoroquinolones could in some cases select for further reductions in drug susceptibility, even in the absence of further exposure to the drug.
References
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Journal ArticleDOI
Supercoiling of the DNA template during transcription.
Leroy F. Liu,James C. Wang +1 more
TL;DR: The model provides an explanation for the experimentally observed high degree of negative or positive supercoiling of intracellular pBR322 DNA when DNA topoisomerase I or gyrase is respectively inhibited and in prokaryotes and eukaryotes.
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DNA gyrase: an enzyme that introduces superhelical turns into DNA
TL;DR: Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase by purified from Escherichia coli cells, and the final superhelix density of the DNA can be considerably greater than that found in intracellularly super coiled DNA.
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Structure and mechanism of DNA topoisomerase II
TL;DR: The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole that provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints.
Book
Nucleic Acids and Molecular Biology
Fritz Eckstein,David M.J. Lilley +1 more
TL;DR: A wide range of topics are covered, including articles on nucleic acid structure, through their interactions with proteins to the control of gene expressions and the plant kingdom has not been forgotten with articles on development and transposition in plants.
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Nalidixic acid resistance: A second genetic character involved in DNA gyrase activity
TL;DR: The nalA locus is responsible for a second component needed for DNA gyrase activity in addition to the component determined by the previously described locus for resistance to novobiocin and coumermycin (cou), which appears to be involved in the nicking-closing activity required in the supercoiling reaction.