Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.
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...…number AY048744). pKD46 (GenBankt Accession number AY048746; Datsenko and Wanner, 2000) was made by PCR amplification of the Red recombinase genes from phage l and cloning into pKD16, a derivative of INT-ts (Haldimann and Wanner, 2001) carrying araC and araBp from pBAD18 (Guzman et al, 1995)....
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...Auto-induction with arabinose—Expression systems in which transcription is controlled by the pBAD promoter of the arabinose operon have relatively low basal expression, which can make them useful for maintaining and expressing toxic genes [38, 39]....
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...For all library construction methods, chemically competent or electrocompetent Escherichia coli strain JM109(DE3) (for pRSET B ) or LMG194 (for pBAD...
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...Frontiers in Microbiology | Microbiotechnology, Ecotoxicology and Bioremediation April 2014 | Volume 5 | Article 172 | 2...
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...Moreover, the field is always expanding and even after almost 40 years from the first human protein obtained in E. coli (Itakura et al., 1977), there is still much room for improvement....
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...For the dual expression of recombinant proteins using two plasmids, systems with the p15A ori are available (pACYC and pBAD series of plasmids, 10–12 copies per cell; Chang and Cohen, 1978; Guzman et al., 1995)....
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...This is the case of the araPBAD promoter present in the pBAD vectors (Guzman et al., 1995)....
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...However, exponential growth in www.frontiersin.org April 2014 | Volume 5 | Article 172 | 1 complex media leads to densities nowhere near that number....
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References
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