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Topics In Fluorescence Spectroscopy
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Citations
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Journal ArticleDOI
Real-time shape approximation and fingerprinting of single proteins using a nanopore.
Erik Yusko,Brandon R. Bruhn,Olivia M. Eggenberger,Olivia M. Eggenberger,Jared Houghtaling,Jared Houghtaling,Ryan Rollings,Nathan Walsh,Santoshi Nandivada,Mariya A. Pindrus,Adam R. Hall,David Sept,Jiali Li,Devendra S. Kalonia,Michael Mayer,Michael Mayer +15 more
TL;DR: The zeptolitre sensing volume of bilayer-coated solid-state nanopores can be used to determine the approximate shape, volume, charge, rotational diffusion coefficient and dipole moment of individual proteins.
Journal ArticleDOI
Exploring natural silk protein sericin for regenerative medicine: an injectable, photoluminescent, cell-adhesive 3D hydrogel
Zheng Wang,Yeshun Zhang,Jinxiang Zhang,Lei Huang,Jia Liu,Yongkui Li,Guozheng Zhang,Subhas C. Kundu,Lin Wang +8 more
TL;DR: This hydrogel is injectable, permitting its implantation through minimally invasive approaches, and is found to exhibit photoluminescence, enabling bioimaging and in vivo tracking, and demonstrates good elasticity, high porosity, and pH-dependent degradation dynamics, which are advantageous for this sericin hydrogels to serve as a delivery vehicle for cells and therapeutic drugs.
Journal ArticleDOI
A comparison of biophysical characterization techniques in predicting monoclonal antibody stability.
TL;DR: Assays that measure solution-mediated interactions and key molecular characteristics of 9 formulated monoclonal antibody (mAb) therapeutics, to predict their stability behavior show reasonable prediction of accelerated stability, with limited predictive value at 2–8°C stability.
Journal ArticleDOI
The surfactant-induced conformational and activity alterations in Rhizopus niveus lipase.
TL;DR: The effect of nonionic, anionic, cationic, and zwitterionic detergents on the enzymatic activity and structural stability of Rhizopus niveus lipase is reported to be helpful in understanding detergent–lipase interaction in greater detail.
Journal ArticleDOI
Purely Organic Dyes with Thermally Activated Delayed Fluorescence—A Versatile Class of Indicators for Optical Temperature Sensing
TL;DR: In this paper, two classes of organic thermally activated delayed fluorescence (TADF) emitters developed for organic light-emitting diode (OLED) technology, are prepared and investigated for their suitability for optical oxygen and temperature sensing.
References
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Journal ArticleDOI
Real-time shape approximation and fingerprinting of single proteins using a nanopore.
Erik Yusko,Brandon R. Bruhn,Olivia M. Eggenberger,Olivia M. Eggenberger,Jared Houghtaling,Jared Houghtaling,Ryan Rollings,Nathan Walsh,Santoshi Nandivada,Mariya A. Pindrus,Adam R. Hall,David Sept,Jiali Li,Devendra S. Kalonia,Michael Mayer,Michael Mayer +15 more
TL;DR: The zeptolitre sensing volume of bilayer-coated solid-state nanopores can be used to determine the approximate shape, volume, charge, rotational diffusion coefficient and dipole moment of individual proteins.
Journal ArticleDOI
Exploring natural silk protein sericin for regenerative medicine: an injectable, photoluminescent, cell-adhesive 3D hydrogel
Zheng Wang,Yeshun Zhang,Jinxiang Zhang,Lei Huang,Jia Liu,Yongkui Li,Guozheng Zhang,Subhas C. Kundu,Lin Wang +8 more
TL;DR: This hydrogel is injectable, permitting its implantation through minimally invasive approaches, and is found to exhibit photoluminescence, enabling bioimaging and in vivo tracking, and demonstrates good elasticity, high porosity, and pH-dependent degradation dynamics, which are advantageous for this sericin hydrogels to serve as a delivery vehicle for cells and therapeutic drugs.
Journal ArticleDOI
A comparison of biophysical characterization techniques in predicting monoclonal antibody stability.
TL;DR: Assays that measure solution-mediated interactions and key molecular characteristics of 9 formulated monoclonal antibody (mAb) therapeutics, to predict their stability behavior show reasonable prediction of accelerated stability, with limited predictive value at 2–8°C stability.
Journal ArticleDOI
The surfactant-induced conformational and activity alterations in Rhizopus niveus lipase.
TL;DR: The effect of nonionic, anionic, cationic, and zwitterionic detergents on the enzymatic activity and structural stability of Rhizopus niveus lipase is reported to be helpful in understanding detergent–lipase interaction in greater detail.
Journal ArticleDOI
Quantitative analysis of 1300-nm three-photon calcium imaging in the mouse brain
Tianyu Wang,Chunyan Wu,Dimitre G. Ouzounov,Wenchao Gu,Fei Xia,Minsu Kim,Xusan Yang,Melissa R. Warden,Chris Xu +8 more
TL;DR: This analysis presents a translatable model for the optimization of three-photon calcium imaging based on experimentally tractable parameters and quantified the cross-over depth beyond which three- photon microscopy outpeforms two-ph photon microscopy in recording fidelity.