TORC1 inactivation stimulates autophagy of nucleoporin and nuclear pore complexes.
Yui Tomioka,Tetsuya Kotani,Hiromi Kirisako,Yu Oikawa,Yayoi Kimura,Hisashi Hirano,Yoshinori Ohsumi,Hitoshi Nakatogawa +7 more
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TLDR
It is revealed that the nuclear pore complex and nucleoporins are degraded by selective autophagy upon inactivation of Tor kinase complex 1 in Saccharomyces cerevisiae.Abstract:
The mechanisms underlying turnover of the nuclear pore complex (NPC) and the component nucleoporins (Nups) are still poorly understood. In this study, we found that the budding yeast Saccharomyces cerevisiae triggers NPC degradation by autophagy upon the inactivation of Tor kinase complex 1. This degradation largely depends on the selective autophagy-specific factor Atg11 and the autophagy receptor-binding ability of Atg8, suggesting that the NPC is degraded via receptor-dependent selective autophagy. Immunoelectron microscopy revealed that NPCs embedded in nuclear envelope-derived double-membrane vesicles are sequestered within autophagosomes. At least two pathways are involved in NPC degradation: Atg39-dependent nucleophagy (selective autophagy of the nucleus) and a pathway involving an unknown receptor. In addition, we found the interaction between Nup159 and Atg8 via the Atg8-family interacting motif is important for degradation of this nucleoporin not assembled into the NPC. Thus, this study provides the first evidence for autophagic degradation of the NPC and Nups, which we term "NPC-phagy" and "nucleoporinophagy."read more
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Autophagy and Autophagy-Related Diseases: A Review.
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Nuclear accumulation of CHMP7 initiates nuclear pore complex injury and subsequent TDP-43 dysfunction in sporadic and familial ALS
Alyssa N. Coyne,Victoria Baskerville,Benjamin L. Zaepfel,Dennis W. Dickson,Frank Rigo,Frank C. Bennett,C. Patrick Lusk,Jeffrey D. Rothstein +7 more
TL;DR: In this article, the authors show that CHMP7, a critical mediator of NPC quality control, is increased in nuclei of C9orf72 and sporadic ALS induced pluripotent stem cell (iPSC)-derived spinal neurons and postmortem human motor cortex before the emergence of Nup alterations.
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ER-phagy: mechanisms, regulation, and diseases connected to the lysosomal clearance of the endoplasmic reticulum
TL;DR: ER-phagy is dysfunctional in specific human diseases and its regulators are subverted by pathogens, highlighting its crucial role for cell and organism life.
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Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress
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References
More filters
Journal ArticleDOI
NIH Image to ImageJ: 25 years of image analysis
TL;DR: The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.
Journal ArticleDOI
Fiji: an open-source platform for biological-image analysis
Johannes Schindelin,Ignacio Arganda-Carreras,Erwin Frise,Verena Kaynig,Mark Longair,Tobias Pietzsch,Stephan Preibisch,Curtis Rueden,Stephan Saalfeld,Benjamin Schmid,Jean-Yves Tinevez,Daniel J. White,Volker Hartenstein,Kevin W. Eliceiri,Pavel Tomancak,Albert Cardona +15 more
TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
Journal ArticleDOI
A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes.
Carsten Janke,Maria M. Magiera,Nicole Rathfelder,Christof Taxis,Simone Reber,Hiromi Maekawa,Alexandra C. Moreno-Borchart,Georg Doenges,Etienne Schwob,Elmar Schiebel,Michael Knop,Michael Knop +11 more
TL;DR: Using the provided cassettes for N‐ and C‐terminal gene tagging or for deletion of any given gene, a set of only four primers is required, which makes this method very cost‐effective and reproducible.
Journal ArticleDOI
Eaten alive: a history of macroautophagy.
Zhifen Yang,Daniel J. Klionsky +1 more
TL;DR: This review traces the key findings that led to the current molecular understanding of this complex process, which is involved in physiology, development, lifespan and a wide range of diseases, including cancer, neurodegeneration and microbial infection.
Journal ArticleDOI
Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control
Robbie Loewith,Estela Jacinto,Stephan Wullschleger,Anja Lorberg,José L. Crespo,Debora Bonenfant,Wolfgang Oppliger,Paul Jenoe,Michael N. Hall +8 more
TL;DR: Two functionally distinct TOR complexes account for the diversity, specificity, and selective rapamycin inhibition of TOR signaling.
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