Toward a quantitative method for estimating tumour-stroma ratio in breast cancer using polarized light microscopy
Jillian Sprenger,Ciara Murray,Jigar Lad,Blake Jones,Georgia Thomas,Sharon Nofech-Mozes,Mohammadali Khorasani,Alex Vitkin +7 more
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TLDR
In this paper, a method of imaging intra and peri-tumoural stroma based on polarized light microscopy was proposed to quantify the tumour-stroma ratio (TSR) in human breast cancer.Abstract:
The tumour-stroma ratio (TSR) has been explored as a useful source of prognostic information in various cancers, including colorectal, breast, and gastric. Despite research showing potential prognostic utility, its uptake into the clinic has been limited, in part due to challenges associated with subjectivity, reproducibility, and quantification. We have recently proposed a simple, robust, and quantifiable high-contrast method of imaging intra- and peri-tumoural stroma based on polarized light microscopy. Here we report on its use to quantify TSR in human breast cancer using unstained slides from 40 patient samples of invasive ductal carcinoma (IDC). Polarimetric results based on a stromal abundance metric correlated well with pathology designations, showing a statistically significant difference between high- and low-stroma samples as scored by two clinical pathologists. The described polarized light imaging methodology shows promise for use as a quantitative, automatic, and standardizable tool for quantifying TSR, potentially addressing some of the challenges associated with its current estimation.read more
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Polarization and Orbital Angular Momentum of Light in Biomedical Applications: feature issue introduction.
TL;DR: This feature issue is a first attempt to summarize the recognitions achieved in this emerging research field of polarized light and optical angular momentum for practical biomedical applications during the last years.
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Polarimetric biomarkers of peri-tumoral stroma can correlate with 5-year survival in patients with left-sided colorectal cancer
TL;DR: In this paper , the peri-tumoral collagenous stroma architecture in 32 human stage III colorectal cancer (CRC) patient samples was assessed using a novel variant of polarized light microscopy for high-contrast imaging and quantification of unstained histology slides.
Journal ArticleDOI
Toward the development of a polarimetric tool to diagnose the fibrotic human ventricular myocardium
O. A. Nevzorova,Richard Schabas +1 more
TL;DR: A portable, affordable, and accurate linear polarization-based diagnostic tool is developed to measure the degree of linear polarization (DOLP) of the myocardial tissues while working at a wavelength of 850 nm as mentioned in this paper .
Journal ArticleDOI
Polarimetric Images of Biological Tissues Based on the Arrow Decomposition of Mueller Matrices
José J. Gil,Ignacio San José,Mónica Canabal-Carbia,E. Estevez,Emilio González-Arnay,Teresa Garnatje,Juan Campos,Angel Lizana +7 more
TL;DR: In this article , a set of polarimetric observables derived from the arrow decomposition of the Mueller matrix were used for enhancing the visualization of the necrotic areas of a Vitis rupestris leaf.
Journal ArticleDOI
Deciphering the Morphology of Tumor-stromal Features in Invasive Breast Cancer Using Artificial Intelligence.
Nehal M Atallah,Noorul Wahab,Michael Thoss,Shorouk Makhlouf,Asmaa Ibrahim,Ayat Lashen,S. F. Ghannam,Nigel P. Mongan,Mostafa Jahanifar,Simon Graham,Mohsin Bilal,Abhir Bhalerao,Shan E Ahmed Raza,David Snead,F. Minhas,Nasir M. Rajpoot,Emad A. Rakha +16 more
TL;DR: In this article , the clinical significance of stroma-to-tumor ratio (S:TR) and spatial arrangement of stromal cells, tumor cell density, and tumor burden in breast cancer was assessed using deep learning models.
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