Toward inline multiplex biodetection of metals, bacteria, and toxins in water networks: the COMBITOX project.
01 Jan 2017-Environmental Science and Pollution Research (Springer Berlin Heidelberg)-Vol. 24, Iss: 1, pp 1-3
TL;DR: This special issue of Environmental Science and Pollution Research highlights selected papers whose results have been obtained in the course of the COMBITOX project, an interdisciplinary research project aiming at conceiving an inline multiparametric device for the surveillance of water networks using biosensors.
Abstract: This special issue of Environmental Science and Pollution Research highlights selected papers whose results have been obtained in the course of the COMBITOX project. COMBITIOX is an interdisciplinary research project funded by the French National Research Agency (ANR) aiming at conceiving an inline multiparametric device for the surveillance of water networks using biosensors. This device is not intended to fully replace chemical methods, but when compared to analytical chromatographic methodologies, biological sensors can offer rapid and on-site monitoring of even trace levels of targeted compounds (Sun et al. 2015) and can quickly raise the alarm in the event of an accidental or intentional pollution. Numerous developments have been published to improve the sensitivity, specificity, and time response of various biosensors in laboratory conditions (Xiong et al. 2012) (der Meer et al. 2010), but their actual transfer into technological devices for the surveillance of water networks remains at a conceptual level. Thus, the challenge here is to go a step beyond and validate biosensors under real-life field conditions by incorporating them in a single inline detector. During the course of COMBITOX, we could define the interface between the biosensors and a common light detector as well as the physical conditioning of the bioreagents and usage protocol. Our resulting prototype allow the detection of bioavailable toxic compounds as well as microorganisms, impacting human health through the drinking water network or interfering with the biological process of modern wastewater treatment plants. We also plan to propose this system to meet the emerging threats such as bioterrorism. COMBITOX focuses on three families of Bobjects^ to detect: metals (cadmium, mercury, arsenic, nickel, etc.), environmental and/or food toxins, and pathogenic microorganisms. Whole-cell biosensors based on reporter gene under the control of an inducible promoter are used to detect various metals (Hynninen and Virta 2010), the antibody/antigen interaction for toxins (Makaraviciute and Ramanaviciene 2013), and the specific infection of bacteria by bacteriophages for pathogenic microorganisms (Smartt et al. 2012) (Vinay et al. 2015). In all cases, the signal measured is photochemical (fluorescence, bioluminescence, or chemo-luminescence): such a method to transduce the biological recognition is very sensitive and a single photodetector can be used for all biosensors included in the device. The challenge here rather lies in the design and the optimization of the different biological compounds for their use in the field while maintaining a high sensibility and robustness. As a consequence, the different articles presented in this special issue focus on original strategies for the optimization and the adaptation of the three types of biosensors for their use in a semi-autonomous inline water analyzer. In the case of whole-cell biosensors, improvement of the dose-responses Responsible editor: Philippe Garrigues
Citations
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TL;DR: There is an upsurge enthusiasm for utilizing biochar produced from waste-biomass in different fields, to address the most important ecological issues as mentioned in this paper, and a review is focused on an overview of remediating harmful contaminants using biochar.
263 citations
TL;DR: A comprehensive evaluation of major cells immobilization aspects, starting from the definition of the technology and focusing on a critical and technical analysis of the currently used immobilization techniques including adsorption, covalent binding, entrapment and encapsulation is provided.
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193 citations
TL;DR: Amino-functionalized magnetic biochar from wasted peanut hull (MPHC-HDA) with excellent adsorption properties for Cr(VI) was successfully prepared by a mild one-step hydrothermal method in the presence of iron chloride and hexamethylenediamine (HDA), and the physicochemical properties of the peanut hull based biochar, HDA-modified PHC (PHC-PHC), and MPHC-hDA were comparatively characterized by XRD, SEM, FT-IR, VSM, N2 adsorptive/des
156 citations
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134 citations
TL;DR: This review presents a state-of-the-art technology for potential use of algae as a low-cost biosorbent for the removal of HMs from wastewater.
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116 citations
References
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250 citations
"Toward inline multiplex biodetectio..." refers background in this paper
...…under the control of an inducible promoter are used to detect various metals (Hynninen and Virta 2010), the antibody/antigen interaction for toxins (Makaraviciute and Ramanaviciene 2013), and the specific infection of bacteria by bacteriophages for pathogenic microorganisms (Smartt et al. 2012)…...
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TL;DR: It is confirmed that MFC-based biosensors could be used as self-powered portable biosensing devices with great potential in long-term and remote environmental monitoring and monitoring of biochemical oxygen demand and toxicity in the environment.
115 citations
TL;DR: This review highlights the use of phages as pathogen detector interfaces to provide the reader with an up-to-date inventory of phage-based biodetection strategies.
Abstract: Bacteriophages, or phages, are bacterial viruses that can infect a broad or narrow range of host organisms. Knowing the host range of a phage allows it to be exploited in targeting various pathogens. Applying phages for the identification of microorganisms related to food and waterborne pathogens and pathogens of clinical significance to humans and animals has a long history, and there has to some extent been a recent revival in these applications as phages have become more extensively integrated into novel detection, identification, and monitoring technologies. Biotechnological and genetic engineering strategies applied to phages are responsible for some of these new methods, but even natural unmodified phages are widely applicable when paired with appropriate innovative detector platforms. This review highlights the use of phages as pathogen detector interfaces to provide the reader with an up-to-date inventory of phage-based biodetection strategies.
87 citations
"Toward inline multiplex biodetectio..." refers background in this paper
...…of an inducible promoter are used to detect various metals (Hynninen and Virta 2010), the antibody/antigen interaction for toxins (Makaraviciute and Ramanaviciene 2013), and the specific infection of bacteria by bacteriophages for pathogenic microorganisms (Smartt et al. 2012) (Vinay et al. 2015)....
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TL;DR: The goal here is not to present a comprehensive summary of individual metal-specific bioreporters that have been constructed, but rather to express views on the theory and applications and identify some directions for future research and development.
Abstract: Whole-cell bioreporters are living microorganisms that produce a specific, quantifiable output in response to target chemicals. Typically, whole-cell bioreporters combine a sensor element for the substance of interest and a reporter element coding for an easily detectable protein. The sensor element is responsible for recognizing the presence of an analyte. In the case of metal bioreporters, the sensor element consists of a DNA promoter region for a metal-binding transcription factor fused to a promoterless reporter gene that encodes a signal-producing protein. In this review, we provide an overview of specific whole-cell bioreporters for heavy metals. Because the sensing of metals by bioreporter microorganisms is usually based on heavy metal resistance/homeostasis mechanisms, the basis of these mechanisms will also be discussed. The goal here is not to present a comprehensive summary of individual metal-specific bioreporters that have been constructed, but rather to express views on the theory and applications of metal-specific bioreporters and identify some directions for future research and development.
59 citations
"Toward inline multiplex biodetectio..." refers methods in this paper
...Whole-cell biosensors based on reporter gene under the control of an inducible promoter are used to detect various metals (Hynninen and Virta 2010), the antibody/antigen interaction for toxins (Makaraviciute and Ramanaviciene 2013), and the specific infection of bacteria by bacteriophages for…...
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