Transformation of azuki bean by Agrobacterium tumefaciens
01 Jan 2001-Plant Cell Tissue and Organ Culture (Kluwer Academic Publishers)-Vol. 64, Iss: 1, pp 47-54
TL;DR: Stable transformation and regeneration was developed for a grain legume, azuki bean and molecular analysis confirmed the stable integration and expression of foreign genes.
Abstract: Stable transformation and regeneration was developed for a grain legume, azuki bean (Vigna angularis Willd. Ohwi & Ohashi). Two constructs containing the neomycin phosphotransferase II gene (nptII) and either the β-glucuronidase (GUS) gene or the modified green fluorescent protein [sGFP(S65T)] gene were introduced independently via Agrobacterium tumefaciens-mediated transformation. After 2 days of co-cultivation on MS medium supplemented with 100 μM acetosyringone and 10 mg l−1 6-benzyladenine, seedling epicotyl explants were placed on regeneration medium containing 100 mg l−1 kanamycin. Adventitious shoots developing from explant calli were excised onto rooting medium containing 100 mg l−1 kanamycin. Rooted shoots were excised and repeatedly selected on the same medium containing kanamycin. Surviving plants were transferred to soil and grown in a green house to produce viable seeds. This process took 5 to 7 months after co-cultivation. Molecular analysis confirmed the stable integration and expression of foreign genes.
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TL;DR: Legumes are a large, diverse family ranging from herbaceous annuals to woody perennials that, because of their capacity to fix nitrogen, are essential components in natural and managed terrestrial ecosystems.
Abstract: Legumes are a large, diverse family ranging from herbaceous annuals to woody perennials that, because of their capacity to fix nitrogen, are essential components in natural and managed terrestrial ecosystems. Legumes have been domesticated for the production of food, feed, forage, fiber, industrial
283 citations
TL;DR: This moderately dense linkage map equipped with many SSR markers will be useful for mapping a range of useful traits such as those related to domestication and stress resistance, and will be used to develop advanced backcross lines for high resolution QTL mapping of these traits.
Abstract: To make progress in genome analysis of azuki bean (Vigna angularis) a genetic linkage map was constructed from a backcross population of (V. nepalensis x V. angularis) x V.angularis consisting of 187 individuals. A total of 486 markers-205 simple sequence repeats (SSRs), 187 amplified fragment length polymorphisms (AFLPs) and 94 restriction fragment length polymorphisms (RFLPs) -were mapped onto 11 linkage groups corresponding to the haploid chromosome number of azuki bean. This map spans a total length of 832.1 cM with an average marker distance of 1.85 cM and is the most saturated map for a Vigna species to date. In addition, RFLP markers from other legumes facilitated finding several orthologous linkage groups based on previously published RFLP linkage maps. Most SSR primers that have been developed from SSR-enriched libraries detected a single locus. The SSR loci identified are distributed throughout the azuki bean genome. This moderately dense linkage map equipped with many SSR markers will be useful for mapping a range of useful traits such as those related to domestication and stress resistance. The mapping population will be used to develop advanced backcross lines for high resolution QTL mapping of these traits.
114 citations
Cites background from "Transformation of azuki bean by Agr..."
...It has the best developed transformation systems among Vigna species (Yamada et al. 2001)....
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TL;DR: In this article, a high-quality draft genome sequence of adzuki bean was generated by whole-genome shotgun sequencing, which reached to 450 Mb (83% of the genome) with an N50 of 38 kb and the total scaffold sequences were 466.7 Mb.
Abstract: Adzuki bean (Vigna angularis), an important legume crop, is grown in more than 30 countries of the world. The seed of adzuki bean, as an important source of starch, digestible protein, mineral elements, and vitamins, is widely used foods for at least a billion people. Here, we generated a high-quality draft genome sequence of adzuki bean by whole-genome shotgun sequencing. The assembled contig sequences reached to 450 Mb (83% of the genome) with an N50 of 38 kb, and the total scaffold sequences were 466.7 Mb with an N50 of 1.29 Mb. Of them, 372.9 Mb of scaffold sequences were assigned to the 11 chromosomes of adzuki bean by using a single nucleotide polymorphism genetic map. A total of 34,183 protein-coding genes were predicted. Functional analysis revealed that significant differences in starch and fat content between adzuki bean and soybean were likely due to transcriptional abundance, rather than copy number variations, of the genes related to starch and oil synthesis. We detected strong selection signals in domestication by the population analysis of 50 accessions including 11 wild, 11 semiwild, 17 landraces, and 11 improved varieties. In addition, the semiwild accessions were illuminated to have a closer relationship to the cultigen accessions than the wild type, suggesting that the semiwild adzuki bean might be a preliminary landrace and play some roles in the adzuki bean domestication. The genome sequence of adzuki bean will facilitate the identification of agronomically important genes and accelerate the improvement of adzuki bean.
93 citations
TL;DR: The morphogenic pathway of adventitious shoot regeneration at the ends of Vigna mungo epicotyl segments was determined by ontogenic age, orientation, polarity and position of the explant on mother seedling as well as when the orientation of explants deviates from the normal vertical upright position.
50 citations
References
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TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Abstract: In experiments with tobacco tissue cultured on White's modified medium (basal meditmi hi Tnhles 1 and 2) supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Subse(iueutly it was found Ihiit this jnoniotiou oi' f^rowih was due mainly though nol entirely to inorj^auic rather than organic con.stitttenls in the extract. In the isolation of Rrowth factors from plant tissues and other sources inorj '̂anic salts are fre(|uently carried along with fhe organic fraclioits. When tissue cultures are used for bioassays, therefore, il is necessary lo lake into account increases in growth which may result from nutrient elements or other known constituents of the medium which may he present in the te.st materials. To minimize interference trom rontaminaitis of this type, an altempt has heen made to de\\eh)p a nieditmi with such adequate supplies of all re(iuired tnineral nutrients and cotntnott orgattic cottslitueitls that no apprecial»le change in growth rate or yield will result from the inlroduclion of additional amounts in the range ordinarily expected to be present in tnaterials to be assayed. As a point of referetice for this work some of the culture media in mc)st common current use will he cotisidered briefly. For ease of comparis4)n Iheir mineral compositions are listed in Tables 1 and 2. White's nutrient .solution, designed originally for excised root cultures, was based on Uspeuski and Uspetiskaia's medium for algae and Trelease and Trelease's micronutrieni solution. This medium also was employed successfully in the original cttltivation of callus from the tobacco Iiybrid Nicotiana gtauca x A', tanijadorffii, atitl as further modified by White in 194̂ ^ and by others it has been used for the
63,098 citations
01 Jan 1962
16,251 citations
"Transformation of azuki bean by Agr..." refers methods in this paper
...They were plated onto basal Murashige and Skoog (MS) medium (Murashige and Skoog, 1962) containing 30 g l−1 sucrose and 8 g l −1agar (Wako Pure Chemical Industries) (pH 5....
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...They were plated onto basal Murashige and Skoog (MS) medium (Murashige and Skoog, 1962) containing 30 g l−1 sucrose and 8 g l−1agar (Wako Pure Chemical Industries) (pH 5.8)....
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TL;DR: Gene fusions can be defined its DNA constructions that result in the coding sequences from one gene (r@o,ter) being transcribed and/or translated under the direction of the controlling sequences of another gene (cmltrr).
Abstract: DeJi~eitio, r Ge~e lrlt.~irm Much of tile attention and interest in modern molecular biology is fi~cussed on the regulation of gene expression. Factors influencing or mediating such regulation are often best studied using gene Alsions. Gene fusions can be defined its DNA constructions (perfi3rmed ill vitro or i~e Hvo) that result in the coding sequences from one gene (r@o,ter) being transcribed and/or translated under the direction of the controlling sequences of another gene (cmltrr Gene fusions can be of two general types, with many wtriatiuns within types. Transcriptional fusions are defined as fusions in which all protein coding sequences are derived from the reporter, with none from the cmm,//e~. Thus, although the m R N A produced may consist of sequences from both control/o and re/;o~ter, the protein synthesized will be encoded only by the reporter. Translational fusions, in contrast, are defined as those in which the polypeptide produced is the result of coding information provided by both copraoiler and reporter.
4,518 citations
TL;DR: A large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with Agrobacterium tumefaciens, and sequence analysis revealed that the boundaries of the T-DNA in transgenic Rice plants were essentially identical to those intransgenic dicotyledons.
Abstract: Summary
A large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with Agrobacterium tumefaciens The efficiency of transformation was similar to that obtained by the methods used routinely for transformation of dicotyledons with the bacterium Stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants in the R0, R1 and R2 generations Sequence analysis revealed that the boundaries of the T-DNA in transgenic rice plants were essentially identical to those in transgenic dicotyledons Calli induced from scutella were very good starting materials A strain of A tumefaciens that carried a so-called ‘super-binary’ vector gave especially high frequencies of transformation of various cultivars of japonica rice that included Koshihikari, which normally shows poor responses in tissue culture
3,475 citations
TL;DR: It is reported that an extensively modified GFP is a versatile and sensitive reporter in a variety of living plant cells and in transgenic plants, and the codon usage effect might be universal, allowing the design of recombinant proteins with high expression efficiency in evolutionarily distant species such as humans and maize.
1,426 citations
"Transformation of azuki bean by Agr..." refers background or methods in this paper
...The presence of sGFP(S65T) was detected by blue light excitation (Chiu et al., 1996)....
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...To construct the second binary vector, we excised a fragment from the insert of pTH-2 harboring the modified green-fluorescent protein gene sGFP(S65T) linked to the CaMV35S promoter and NOS terminator (Chiu et al., 1996), by digestion ofHindIII and EcoRI....
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