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Journal ArticleDOI

Transient state analysis of biochemical reactor using coimmobilized system

01 Feb 1994-Bioprocess Engineering (Springer Nature)-Vol. 10, Iss: 2, pp 83-89
TL;DR: In this paper, the transient state analysis of the consecutive sequence of reactions S → P1 → P2 taking place inside a porous spherical co-immobilized biocatalyst is discussed for the case in which each step follows Michaelis Menten type kinetics.
Abstract: The transient state analysis of the consecutive sequence of reactions S → P1 → P2 taking place inside a porous spherical coimmobilized biocatalyst is discussed for the case in which each step follows Michaelis Menten type kinetics. The theoretical analysis includes intraparticle diffusional limitations. The model equations are solved by the explicit finite difference method. The effect of various parameters of importance on the batch reactor performance is discussed. Comparison of the model with experimental results has been shown.
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25,389 citations


"Transient state analysis of biochem..." refers methods in this paper

  • ...Reducing sugars were estimated by dinitrosalicylic acid method [14] Ethanol was estimated by the gas chromatographic method using the activated chromosorb 10t (80/100 mesh) column with flame ionization detector [15]....

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Journal ArticleDOI
TL;DR: The rate of production of the end product at the first stages of the reaction is markedly higher in the immobilized enzyme system than that predicted for a corresponding homogeneous system.

132 citations


"Transient state analysis of biochem..." refers background in this paper

  • ...Goldman and Katchalski [ 10 ] have theoretically analysed the kinetic behaviour of a two enzyme system carrying out a consecutive reaction where the enzymes are impregnated on an impermeable flat membrane....

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Journal ArticleDOI
TL;DR: The production of ethanol from starch by a coimmobilized mixed culture system of aerobic and anaerobic microorganisms in Ca‐alginate gel beads was investigated and the advantages of the system were clarified.
Abstract: The production of ethanol from starch by a coimmobilized mixed culture system of aerobic and anaerobic microorganisms in Ca-alginate gel beads was investigated. The mold Aspergillus awamori was used as an aerobic amylolytic microorganism and an anaerobic bacterium, Zymomonas mobilis, as an ethanol producer. By controlling the mixing ratio of the microorganisms in the inoculum size, a desirable coimmobilized mixed culture system, in which the aerobic mycelia grew on and near the oxygen-rich surface of the gel beads while the anaerobic bacterial cells mainly grew in the oxygen-deficient central part of the gel beads, was naturally established under the aerobic culture conditions, and ethanol could be directly produced from starch by the system. The ethanol productivity by the system in flask culture was particularly affected by the shear stress (dependent on the shaking speed) which controlled the mycelial growth on the surface of the gel beads. Under optimum culture conditions in the flask culture, the glucose produced was instantly consumed, and was not observed in the culture broth; the final concentration of ethanol produced from 100 g/L starch was 25 g/L and the yield coefficient for ethanol, Y/sub p/s/, was 0.38. The ethanol productivity by the coimmobilized mixed culture systemmore » was compared with those by other various culture systems and the advantages of the system were clarified.« less

115 citations