Ts2631 Endolysin from the Extremophilic Thermus scotoductus Bacteriophage vB_Tsc2631 as an Antimicrobial Agent against Gram-Negative Multidrug-Resistant Bacteria.
Magdalena Plotka,Małgorzata Kapusta,Sebastian Dorawa,Anna-Karina Kaczorowska,Tadeusz Kaczorowski +4 more
Reads0
Chats0
TLDR
The results indicate that Ts2631 endolysin could be useful to combat Gram-negative pathogens and is demonstrated to be dependent on the presence of its N-terminal tail.Abstract:
Bacteria that thrive in extreme conditions and the bacteriophages that infect them are sources of valuable enzymes resistant to denaturation at high temperatures. Many of these heat-stable proteins are useful for biotechnological applications; nevertheless, none have been utilized as antibacterial agents. Here, we demonstrate the bactericidal potential of Ts2631 endolysin from the extremophilic bacteriophage vB_Tsc2631, which infects Thermus scotoductus, against the alarming multidrug-resistant clinical strains of Acinetobacter baumannii, Pseudomonas aeruginosa and pathogens from the Enterobacteriaceae family. A 2–3.7 log reduction in the bacterial load was observed in antibacterial tests against A. baumannii and P. aeruginosa after 1.5 h. The Ts2631 activity was further enhanced by ethylenediaminetetraacetic acid (EDTA), a metal ion chelator (4.2 log reduction in carbapenem-resistant A. baumannii) and, to a lesser extent, by malic acid and citric acid (2.9 and 3.3 log reductions, respectively). The EDTA/Ts2631 combination reduced all pathogens of the Enterobacteriaceae family, particularly multidrug-resistant Citrobacter braakii, to levels below the detection limit (>6 log); these results indicate that Ts2631 endolysin could be useful to combat Gram-negative pathogens. The investigation of A. baumannii cells treated with Ts2631 endolysin variants under transmission electron and fluorescence microscopy demonstrates that the intrinsic antibacterial activity of Ts2631 endolysin is dependent on the presence of its N-terminal tail.read more
Citations
More filters
Journal ArticleDOI
Antimicrobial Peptides: An Update on Classifications and Databases
TL;DR: In this paper, the authors summarize the sources, structures, modes of action, and classifications of AMPs and compare valuable computational tools used to predict antimicrobial activity and mechanisms of action.
Journal ArticleDOI
Exploiting phage receptor binding proteins to enable endolysins to kill Gram-negative bacteria.
Athina Zampara,Martine C. Holst Sørensen,Dennis Grimon,Fabio Antenucci,Amira Ruslanovna Vitt,Valeria Bortolaia,Yves Briers,Lone Brøndsted +7 more
TL;DR: The Innolysin approach expands previous endolysin-engineering strategies, allowing customization of endolysins by exploiting phage RBPs to specifically target Gram-negative bacteria.
Journal ArticleDOI
Endolysin, a Promising Solution against Antimicrobial Resistance.
Mujeeb ur Rahman,Weixiao Wang,Qingqing Sun,Junaid Ali Shah,Chao Li,Yanmei Sun,Yuanrui Li,Bailing Zhang,Wei Chen,Shiwei Wang +9 more
TL;DR: This review comprehensively introduces the structures and activities of endolysins and summarize the latest application progress of recombinant endolySins in the fields of medical treatment, pathogen diagnosis, food safety, and agriculture.
Journal ArticleDOI
Bacteriophage-encoded enzymes destroying bacterial cell membranes and walls, and their potential use as antimicrobial agents.
Łukasz Grabowski,Krzysztof Łepek,Małgorzata Stasiłojć,Katarzyna Kosznik-Kwaśnicka,Karolina Zdrojewska,Monika Maciąg-Dorszyńska,Grzegorz Węgrzyn,Alicja Węgrzyn +7 more
TL;DR: In this article, the authors present and discuss the current knowledge on the processes of bacteriophage-mediated bacterial cell lysis, with special emphasis on enzymes involved in them, and possibilities of the practical use of these enzymes as antibacterial agents are underlined and perspectives of this aspect will be presented.
Journal ArticleDOI
Lysins breaking down the walls of Gram-negative bacteria, no longer a no-go.
Diana Gutiérrez,Yves Briers +1 more
TL;DR: Phage lysins are one of the most advanced classes of antibacterials under clinical evaluation and have a new mode of action based on peptidoglycan degradation and have been recently explored and advanced to different extents.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability
Claire Vieille,Gregory J. Zeikus +1 more
TL;DR: This review concentrates on the remarkable thermostability of hyperthermophilic enzymes, and describes the biochemical and molecular properties of these enzymes, which are typically thermostable and optimally active at high temperatures.
Journal ArticleDOI
Peptidoglycan structure and architecture
TL;DR: In several species examined, the fine structure of the peptidoglycan significantly varies with the growth conditions, and the different models for the architecture are discussed with respect to structural and physical parameters.
Journal ArticleDOI
Antibiotics and Bacterial Resistance in the 21st Century
Richard J. Fair,Yitzhak Tor +1 more
TL;DR: In this review the factors that have been linked to the waxing of bacterial resistance are addressed and profiles of bacterial species that are deemed to be particularly concerning at the present time are illustrated.
Journal ArticleDOI
Challenges of Antibacterial Discovery
TL;DR: The purpose of this review is to underscore and illustrate those scientific problems unique to the discovery and optimization of novel antibacterial agents that have adversely affected the output of the effort.
Related Papers (5)
Bacteriophage endolysins as novel antimicrobials
Potential for Bacteriophage Endolysins to Supplement or Replace Antibiotics in Food Production and Clinical Care.
Breaking barriers: expansion of the use of endolysins as novel antibacterials against Gram-negative bacteria
Yves Briers,Rob Lavigne +1 more