scispace - formally typeset
Search or ask a question
Patent

Two phase extraction of oil from biomass

15 Dec 1998-
TL;DR: In this paper, a method of separating edible oil from biological material is disclosed, where a biomass slurry containing microbial material in an aqueous suspension is collected, typically placed in a centrifuge and then in a homogenizer.
Abstract: A method of separating edible oil from biological material is disclosed. A biomass slurry containing microbial material in an aqueous suspension is collected. The slurry is typically placed in a centrifuge and then in a homogenizer. The resulting slurry is fed into a contacting device, such as a packed column, and mixed with a solvent that is essentially immiscible in water, for example hexane. The solvent extracts the oil from the biomass slurry and then separates from the slurry. Edible oil is recovered from the solvent and further processed.
Citations
More filters
Journal ArticleDOI
TL;DR: Various microbiological and enzymatic methods for synthesis of PUFAs are discussed and marine protists and dinoflagellates are the rich sources of DHA, whereas microalgae like Phaeodactylum and Monodus are good sources of EPA.

747 citations

Journal ArticleDOI
TL;DR: This paper aims to present a critical review on lipid recovery in the context of biofuel productions with special attention to cell disruption and lipid mass transfer to support extraction from wet biomass.

250 citations

Patent
19 Jan 2001
TL;DR: In this article, a method for extracting lipids from microorganisms without using a nonpolar organic solvent as an extraction solvent is presented, using aqueous washing solutions until a substantially non-emulsified lipid is obtained.
Abstract: The present invention provides a method for extracting lipids from microorganisms without using a nonpolar organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

205 citations

Patent
02 Jun 2008
TL;DR: In this paper, the authors provide methods and compositions useful for the production of oil, fuels, oleochemicals, and other compounds in microorgansims, including methods of low-cost cultivation of such microorganisms.
Abstract: The invention provides methods and compositions useful for the production of oil, fuels, oleochemicals, and other compounds in microorgansims. In particular, the invention provides oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. The invention also provides microbial cells containing exogenous genes encoding, for example, a lipase, sucrose transporter, sucrose invertase, fructokinase, a polysaccharide-degrading enzyme, fatty acyl-ACP thioesterase, fatty acyl-CoA/aldehyde reductase, fatty acyl-CoA reductase, fatty aldehyde reductase, fatty aldehyde decarbonylase, and/or an acyl carrier protein. The invention also includes methods of manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel.

172 citations

Patent
20 Mar 2006
TL;DR: In this paper, a system for producing engineered oleaginous yeast or fungi that express carotenoids was presented, which can be used to produce carotenes. But this system is not suitable for agricultural applications.
Abstract: The present invention provides systems for producing engineered oleaginous yeast or fungi that express carotenoids.

155 citations

References
More filters
Patent
12 Oct 1993
TL;DR: In this article, a process for growing the microflora Thraustochytrium, Schizchytrium and mixtures thereof, which includes the growing of the micro-flora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate, was described.
Abstract: Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.

361 citations

Patent
TL;DR: The present invention relates to compositions including blends of microbial oils, methods of using such compositions, particularly as supplements for infant formula, and methods of increasing the amount of long chain polyunsaturated fatty acids in infant formula as mentioned in this paper.
Abstract: The present invention relates to compositions including blends of microbial oils, methods of using such compositions, particularly as supplements for infant formula, and methods of increasing the amount of long chain polyunsaturated fatty acids in infant formula.

255 citations

Patent
03 Jan 1996
TL;DR: In this article, a process for the production of arachidonic acid containing oils, which preferably are substantially free of eicosapentaneoic acid, is described.
Abstract: The present invention relates to processes for the production of arachidonic acid containing oils, which preferably are substantially free of eicosapentaneoic acid. The invention also relates to compositions containing oils having very high amounts of arachidonic acid in triglyceride form, and to uses of such oils. In a preferred embodiment, Mortierella alpina is cultivated using conditions which yield triglyceride oil having particularly high levels of arachidonic acid residues, biomass is harvested and the oil is extracted, recovered, and used as an additive for infant formula.

227 citations

Patent
04 Feb 1991
TL;DR: In this paper, a single cell edible oil from dinoflagellates was used to produce infant formula and baby food compositions which contained at least 70% triglycerides which contain about 20-35% docosahexaenoic acid (DHA) and lack eicosapentaenoic acids (EPA).
Abstract: Infant formula and baby food compositions are presented which contain single cell edible oil which is recovered from dinoflagellates and which lacks unpleasant tastes and fishy odors. This single cell edible oil comprises at least 70% triglycerides which contain about 20-35% docosahexaenoic acid (DHA) and lack eicosapentaenoic acid (EPA). To produce the single cell oil, the dinoflagellates are cultivated in fermentors and induced to produce the single cell oil which is subsequently recovered by extraction with solvents.

143 citations

Patent
25 Jan 1993
TL;DR: In this article, a process for the selective production of polyunsaturated fatty acids from a culture of microalgae of Porphyrium cruentum is described, which is based on growing a biomass by culturing the micro algae in a photobioreactor under optimum culturing conditions, removing a portion of the resulting biomass, and subjecting the removed portion to a temperature decrease.
Abstract: The invention relates to a process for the selective production of polyunsaturated fatty acids from a culture of microalgae of Porphyrium cruentum. The process comprises growing a biomass by culturing the microalgae in a photobioreactor under optimum culturing conditions, removing a portion of the resulting biomass, and subjecting the removed portion to a temperature decrease. The result is the selective increase in the quantity of a given polyunsaturated fatty acid within the microalgae biomass.

137 citations