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Two-photon laser scanning fluorescence microscopy and its applications
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The principle of two-photon laser scanning fluorescence microscopy and its applications are reviewed in this article, where the intrinsic three dimensional spatial resolution has been widely exploited in the life sciences,semiconductor technology,optical data storage and lithographic microfabrication.Abstract:
The principle of two-photon laser scanning fluorescence microscopy and its applications are reviewed.Its intrinsic three dimensional spatial resolution has been widely exploited in the life sciences,semiconductor technology,optical data storage and lithographic microfabrication.read more
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Proceedings ArticleDOI
Restoration of fluorescence images from two-photon microscopy using modified nonlinear anisotropic diffusion filter
TL;DR: Wang et al. as discussed by the authors proposed a modified nonlinear anisotropic diffusion filter which incorporates both gradient and gray-level variance of raw data, to remove the noise, rather than merely considering gradient as the classical Perona-Malik nonlinear diffusion model.
Journal ArticleDOI
Super-resolution Imaging by Two-photon Structured Illumination Microscopy
TL;DR: In this paper, a nonlinear two-photon structured illumination microscopy (NTPSIM) was proposed, which combines SIM with twophoton excitation, enabling resolution in thick specimens imaging twice of the normal resolution.
References
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Journal ArticleDOI
Super-resolution Imaging by Two-photon Structured Illumination Microscopy
TL;DR: In this paper, a nonlinear two-photon structured illumination microscopy (NTPSIM) was proposed, which combines SIM with twophoton excitation, enabling resolution in thick specimens imaging twice of the normal resolution.