Figure 6. Fluorescently labeled Drosophila chromosome imaged by wide-field deconvolution microscopy (M. Lowenstein and J. Sedat, UCSF, unpublished data). Three fluorophores were used to label specific segments of chromosome 2L. Two homologous copies of the chromosome are shown. The cyan isosurface is the nuclear envelope. The individual fluorescent spots have been marked with the Volume Path Tracer extension and paths connecting the markers are shown as smooth tubes. Traced structures from many cells can be clustered to study structural patterns of chromosome organization in the nucleus.
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