Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor.
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TLDR
In this paper, a laser-induced graphene field effect transistor (LIG-FET) was used for detecting SARS-CoV-2 spike protein in 15 min at a concentration of 1 pg/mL in phosphate-buffered saline (PBS) and 1 ng/mL of human serum.Abstract:
COVID-19 is a highly contagious human infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the war with the virus is still underway. Since no specific drugs have been made available yet and there is an imbalance between supply and demand for vaccines, early diagnosis and isolation are essential to control the outbreak. Current nucleic acid testing methods require high sample quality and laboratory conditions, which cannot meet flexible applications. Here, we report a laser-induced graphene field-effect transistor (LIG-FET) for detecting SARS-CoV-2. The FET was manufactured by different reduction degree LIG, with an oyster reef-like porous graphene channel to enrich the binding point between the virus protein and sensing area. After immobilizing specific antibodies in the channel, the FET can detect the SARS-CoV-2 spike protein in 15 min at a concentration of 1 pg/mL in phosphate-buffered saline (PBS) and 1 ng/mL in human serum. In addition, the sensor shows great specificity to the spike protein of SARS-CoV-2. Our sensors can realize fast production for COVID-19 rapid testing, as each LIG-FET can be fabricated by a laser platform in seconds. It is the first time that LIG has realized a virus sensing FET without any sample pretreatment or labeling, which paves the way for low-cost and rapid detection of COVID-19.read more
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Journal ArticleDOI
Recent Advances in Laser‐Induced Graphene: Mechanism, Fabrication, Properties, and Applications in Flexible Electronics
Truong-Son Dinh Le,Hoang‐Phuong Phan,Soongeun Kwon,Sangbaek Park,Yeong-Tae Jung,Jin Min,Byung Jae Chun,Hana Yoon,Seung Hwan Ko,Seungchul Kim,Young-Jin Kim +10 more
TL;DR: Laser-induced graphene (LIG) as mentioned in this paper is a newly emerging 3D porous material produced when irradiating a laser beam on certain carbon materials, which exhibits high porosity, excellent electrical conductivity, and good mechanical flexibility.
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Aerosol-jet-printed graphene electrochemical immunosensors for rapid and label-free detection of SARS-CoV-2 in saliva
Cícero C. Pola,Sonal V. Rangnekar,Robert Leonard Sheets,Beata M. Szydłowska,Julia R. Downing,Kshama Parate,Shay G. Wallace,Da-Shiang Tsai,Mark C. Hersam,Carmen L. Gomes,Jonathan C. Claussen +10 more
TL;DR: This work employs custom-formulated graphene inks and aerosol jet printing to create a rapid electrochemical immunosensor for direct detection of SARS-CoV-2 spike receptor-binding domain (RBD) in saliva samples acquired noninvasively.
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Laser-Induced Graphene (LIG) as a Smart and Sustainable Material to Restrain Pandemics and Endemics: A Perspective
Nandini Dixit,Swatantra P. Singh +1 more
TL;DR: Laser-induced graphene (LIG) is a three-dimensional carbon-based nanomaterial fabricated in a single step on a wide variety of low-cost to high-quality carbonaceous materials without using any additional chemicals potentially used for antiviral, antibacterial, and sensing applications as mentioned in this paper .
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Microfluidics-Based Biosensing Platforms: Emerging Frontiers in Point-of-Care Testing SARS-CoV-2 and Seroprevalence
Elda A. Flores-Contreras,Reyna Berenice González-González,Iram P. Rodriguez-Sanchez,Juan F. Yee-de León,Hafiz M.N. Iqbal,Everardo González-González +5 more
TL;DR: This review discusses the different microfluidic platforms applied in detecting SARS-CoV-2 and seroprevalence, classified into three sections according to the molecules to be detected, i.e., nucleic acid, antigens, and anti-SARS- CoV- 2 antibodies.
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The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review
TL;DR: The role played by electrochemical biosensors in SARS-CoV-2 detection from a bibliometric perspective was analyzed in this paper , where the contributions of different countries and institutions to this topic were analyzed.
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