Ultrasensitive fluorescent proteins for imaging neuronal activity.
Tsai Wen Chen,Trevor J. Wardill,Trevor J. Wardill,Yi Sun,Stefan R. Pulver,Sabine L. Renninger,Amy Baohan,Amy Baohan,Eric R. Schreiter,Rex Kerr,Michael B. Orger,Vivek Jayaraman,Loren L. Looger,Karel Svoboda,Douglas S. Kim +14 more
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TLDR
A family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo are developed and provide new windows into the organization and dynamics of neural circuits over multiple spatial and temporal scales.Abstract:
Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo. In layer 2/3 pyramidal neurons of the mouse visual cortex, GCaMP6 reliably detected single action potentials in neuronal somata and orientation-tuned synaptic calcium transients in individual dendritic spines. The orientation tuning of structurally persistent spines was largely stable over timescales of weeks. Orientation tuning averaged across spine populations predicted the tuning of their parent cell. Although the somata of GABAergic neurons showed little orientation tuning, their dendrites included highly tuned dendritic segments (5-40-µm long). GCaMP6 sensors thus provide new windows into the organization and dynamics of neural circuits over multiple spatial and temporal scales.read more
Citations
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Neuronal circuits for fear and anxiety
TL;DR: This Review focuses on studies that have used circuit-based approaches to gain a more detailed, and also more comprehensive and integrated, view on how the brain governs fear and anxiety and how it orchestrates adaptive defensive behaviours.
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Natural Neural Projection Dynamics Underlying Social Behavior
Lisa A. Gunaydin,Logan Grosenick,Joel Finkelstein,Isaac Kauvar,Lief E. Fenno,Avishek Adhikari,Stephan Lammel,Julie J. Mirzabekov,Raag D. Airan,Kelly A. Zalocusky,Kay M. Tye,Polina Anikeeva,Robert C. Malenka,Karl Deisseroth,Karl Deisseroth +14 more
TL;DR: Fiber photometry was developed and applied to optically record natural neural activity in genetically and connectivity-defined projections to elucidate the real-time role of specified pathways in mammalian behavior and captures a fundamental and previously inaccessible dimension of mammalian circuit dynamics.
Journal ArticleDOI
Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Linda Madisen,Aleena R. Garner,Daisuke Shimaoka,Amy S. Chuong,Nathan C. Klapoetke,Lu Li,Alexander van der Bourg,Yusuke Niino,Ladan Egolf,Claudio Monetti,Hong Gu,Maya Mills,Adrian Cheng,Bosiljka Tasic,Thuc Nghi Nguyen,Susan M. Sunkin,Andrea Benucci,Andras Nagy,Atsushi Miyawaki,Fritjof Helmchen,Ruth M. Empson,Thomas Knöpfel,Edward S. Boyden,R. Clay Reid,Matteo Carandini,Hongkui Zeng +25 more
TL;DR: These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity, and develop driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems.
Journal ArticleDOI
A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons.
D. Gowanlock R. Tervo,Bum Yeol Hwang,Sarada Viswanathan,Thomas Gaj,Maria Lavzin,Maria Lavzin,Kimberly D. Ritola,Sarah Lindo,Susan Michael,Elena Kuleshova,Elena Kuleshova,David Stephen Ojala,Cheng Chiu Huang,Charles R. Gerfen,Charles R. Gerfen,Jackie Schiller,Jackie Schiller,Joshua T. Dudman,Adam W. Hantman,Loren L. Looger,David V. Schaffer,Alla Y. Karpova +21 more
TL;DR: A newly evolved variant of adeno-associated virus, rAAV2-retro, permits robust retrograde access to projection neurons with efficiency comparable to classical synthetic retrograde tracers and enables sufficient sensor/effector expression for functional circuit interrogation and in vivo genome editing in targeted neuronal populations.
Journal ArticleDOI
Simultaneous Denoising, Deconvolution, and Demixing of Calcium Imaging Data
Eftychios A. Pnevmatikakis,Daniel Soudry,Yuanjun Gao,Timothy A. Machado,Josh Merel,David Pfau,Thomas Reardon,Thomas Reardon,Yu Mu,Clay Lacefield,Weijian Yang,Misha B. Ahrens,Randy M. Bruno,Thomas M. Jessell,Thomas M. Jessell,Darcy S. Peterka,Rafael Yuste,Liam Paninski +17 more
TL;DR: This work presents a modular approach for analyzing calcium imaging recordings of large neuronal ensembles that relies on a constrained nonnegative matrix factorization that expresses the spatiotemporal fluorescence activity as the product of a spatial matrix that encodes the spatial footprint of each neurons in the optical field and a temporal matrix that characterizes the calcium concentration of each neuron over time.
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