Open Access
Universal sample preparation method for proteome analysis
Reads0
Chats0
About:
The article was published on 2009-01-01 and is currently open access. It has received 1586 citations till now.read more
Citations
More filters
Journal ArticleDOI
Deep proteome and transcriptome mapping of a human cancer cell line
Nagarjuna Nagaraj,Jacek R. Wisniewski,Tamar Geiger,Juergen Cox,Martin Kircher,Janet Kelso,Svante Pääbo,Matthias Mann +7 more
TL;DR: Comparisons of the proteome and the transcriptome, and analysis of protein complex databases and GO categories, suggest that deep coverage of the functional transcriptome andThe proteome of a single cell type is achieved.
Journal ArticleDOI
C9orf72 nucleotide repeat structures initiate molecular cascades of disease
Aaron R. Haeusler,Christopher J. Donnelly,Goran Periz,Eric A J Simko,Patrick G. Shaw,Min-Sik Kim,Nicholas J. Maragakis,Juan C. Troncoso,Akhilesh Pandey,Rita Sattler,Jeffrey D. Rothstein,Jiou Wang +11 more
TL;DR: It is demonstrated that distinct C9orf72 HRE structural polymorphism at both DNA and RNA levels initiates molecular cascades leading to ALS/FTD pathologies, and provides the basis for a mechanistic model for repeat-associated neurodegenerative diseases.
Journal ArticleDOI
Precision Mapping of an In Vivo N-Glycoproteome Reveals Rigid Topological and Sequence Constraints
TL;DR: A "filter aided sample preparation" (FASP)-based method in which glycopeptides are enriched by binding to lectins on the top of a filter and mapped 6367 N-glycosylation sites on 2352 proteins in four mouse tissues and blood plasma using high-accuracy mass spectrometry reveals that the sites always orient toward the extracellular space or toward the lumen of ER, Golgi, lysosome, or peroxisome.
Journal ArticleDOI
A census of human soluble protein complexes.
Pierre C. Havugimana,G. Traver Hart,Tamás Nepusz,Haixuan Yang,Andrei L. Turinsky,Zhihua Li,Peggy I. Wang,Daniel R. Boutz,Vincent Fong,Sadhna Phanse,Mohan Babu,Stephanie A. Craig,Pingzhao Hu,Cuihong Wan,James Vlasblom,Vaqaar Un Nisa Dar,Alexandr Bezginov,Greg W. Clark,Gabriel C. Wu,Shoshana J. Wodak,Elisabeth R. M. Tillier,Alberto Paccanaro,Edward M. Marcotte,Andrew Emili +23 more
TL;DR: Whereas larger multiprotein assemblies tend to be more extensively annotated and evolutionarily conserved, human protein complexes with five or fewer subunits are far more likely to be functionally unannotated or restricted to vertebrates, suggesting more recent functional innovations.
Journal ArticleDOI
The quantitative proteome of a human cell line
Martin Beck,Alexander Schmidt,Johan Malmstroem,Manfred Claassen,Alessandro Ori,Anna Szymborska,Franz Herzog,Oliver Rinner,Jan Ellenberg,Ruedi Aebersold,Ruedi Aebersold +10 more
TL;DR: This work provides a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis, and shows that these human cultured cells express at least ∼10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell.
References
More filters
Journal ArticleDOI
Deep proteome and transcriptome mapping of a human cancer cell line
Nagarjuna Nagaraj,Jacek R. Wisniewski,Tamar Geiger,Juergen Cox,Martin Kircher,Janet Kelso,Svante Pääbo,Matthias Mann +7 more
TL;DR: Comparisons of the proteome and the transcriptome, and analysis of protein complex databases and GO categories, suggest that deep coverage of the functional transcriptome andThe proteome of a single cell type is achieved.
Journal ArticleDOI
C9orf72 nucleotide repeat structures initiate molecular cascades of disease
Aaron R. Haeusler,Christopher J. Donnelly,Goran Periz,Eric A J Simko,Patrick G. Shaw,Min-Sik Kim,Nicholas J. Maragakis,Juan C. Troncoso,Akhilesh Pandey,Rita Sattler,Jeffrey D. Rothstein,Jiou Wang +11 more
TL;DR: It is demonstrated that distinct C9orf72 HRE structural polymorphism at both DNA and RNA levels initiates molecular cascades leading to ALS/FTD pathologies, and provides the basis for a mechanistic model for repeat-associated neurodegenerative diseases.
Journal ArticleDOI
Precision Mapping of an In Vivo N-Glycoproteome Reveals Rigid Topological and Sequence Constraints
TL;DR: A "filter aided sample preparation" (FASP)-based method in which glycopeptides are enriched by binding to lectins on the top of a filter and mapped 6367 N-glycosylation sites on 2352 proteins in four mouse tissues and blood plasma using high-accuracy mass spectrometry reveals that the sites always orient toward the extracellular space or toward the lumen of ER, Golgi, lysosome, or peroxisome.
Journal ArticleDOI
A census of human soluble protein complexes.
Pierre C. Havugimana,G. Traver Hart,Tamás Nepusz,Haixuan Yang,Andrei L. Turinsky,Zhihua Li,Peggy I. Wang,Daniel R. Boutz,Vincent Fong,Sadhna Phanse,Mohan Babu,Stephanie A. Craig,Pingzhao Hu,Cuihong Wan,James Vlasblom,Vaqaar Un Nisa Dar,Alexandr Bezginov,Greg W. Clark,Gabriel C. Wu,Shoshana J. Wodak,Elisabeth R. M. Tillier,Alberto Paccanaro,Edward M. Marcotte,Andrew Emili +23 more
TL;DR: Whereas larger multiprotein assemblies tend to be more extensively annotated and evolutionarily conserved, human protein complexes with five or fewer subunits are far more likely to be functionally unannotated or restricted to vertebrates, suggesting more recent functional innovations.
Journal ArticleDOI
The quantitative proteome of a human cell line
Martin Beck,Alexander Schmidt,Johan Malmstroem,Manfred Claassen,Alessandro Ori,Anna Szymborska,Franz Herzog,Oliver Rinner,Jan Ellenberg,Ruedi Aebersold,Ruedi Aebersold +10 more
TL;DR: This work provides a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis, and shows that these human cultured cells express at least ∼10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell.