Urinary 1H-NMR Metabolomics Can Distinguish Pancreatitis Patients from Healthy Controls
Elizabeth R Lusczek,Joao A. Paulo,John R. Saltzman,Vivek Kadiyala,Peter A. Banks,Greg J. Beilman,Darwin L. Conwell +6 more
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TLDR
This metabolomic investigation demonstrates that this non-invasive technique offers insight into the metabolic states of pancreatitis, although the identified metabolites cannot conclusively be defined as biomarkers of disease, future studies will validate the findings in larger patient cohorts.Abstract:
Context The characterization of the urinary metabolome may yield biomarkers indicative of pancreatitis. Objectives We establish a non-invasive technique to compare urinary metabolic profiles in patients with acute and chronic pancreatitis to healthy controls. Methods Urine was obtained from healthy controls (HC, n=5), inpatients with mild acute pancreatitis (AP, n=5), and outpatients with chronic pancreatitis (CP, n=5). Proton nuclear magnetic resonance spectra were obtained for each sample. Metabolites were identified and quantified in each spectrum; resulting concentrations were normalized to account for differences in dilution among samples. Kruskal-Wallis test, post-hoc Mann-Whitney U tests, and principal component analysis were performed to identify metabolites that discriminate healthy controls, acute pancreatitis, and chronic pancreatitis. Results Sixty metabolites were identified and quantified; five were found to differ significantly (P<0.05) among the three groups. Of these, citrate and adenosine remained significant after validation by random permutation. Principal component analysis demonstrated that healthy control urine samples can be differentiated from patients with chronic pancreatitis or acute pancreatitis; chronic pancreatitis patients could not be distinguished from acute pancreatitis patients. Conclusions This metabolomic investigation demonstrates that this non-invasive technique offers insight into the metabolic states of pancreatitis. Although the identified metabolites cannot conclusively be defined as biomarkers of disease, future studies will validate our findings in larger patient cohorts. Image: Boxplots of two significant metabolites.read more
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Recommendations and Standardization of Biomarker Quantification Using NMR-Based Metabolomics with Particular Focus on Urinary Analysis
Abdul-Hamid M. Emwas,Raja Roy,Ryan T. McKay,Danielle Ryan,Lorraine Brennan,Leonardo Tenori,Claudio Luchinat,Xin Gao,Ana Carolina de Mattos Zeri,G. A. Nagana Gowda,Daniel Raftery,Daniel Raftery,Christoph Steinbeck,Reza M. Salek,David S. Wishart +14 more
TL;DR: A number of the most significant issues regarding NMR-based urinary metabolomics with specific emphasis on metabolite quantification for disease biomarker applications are discussed and propose data collection and instrumental recommendations regarding N MR pulse sequences, acceptable acquisition parameter ranges, relaxation effects on quantitation, proper handling of instrumental differences, sample preparation, and biomarker assessment.
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NMR metabolomics of human blood and urine in disease research.
TL;DR: This paper reviews the main applications of NMR metabolomics of blood and urine in disease research, over the last 5 years, to provide insight into underlying disease pathogenesis and unveil new metabolic markers for disease diagnosis and follow up.
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Plasma Metabolite Biomarkers for the Detection of Pancreatic Cancer
Guoxiang Xie,Lingeng Lu,Yunping Qiu,Quanxing Ni,Wei Zhang,Yu-Tang Gao,Harvey A. Risch,Herbert Yu,Wei Jia +8 more
TL;DR: Plasma metabolic signatures show promise as biomarkers for early detection of PC using a metabonomics approach to identify potential markers.
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Volatile organic compounds (VOCs) for the non-invasive detection of pancreatic cancer from urine.
Emma Daulton,Alfian Wicaksono,Akira Tiele,Hemant M. Kocher,Silvana Debernardi,Tatjana Crnogorac-Jurcevic,James A. Covington +6 more
TL;DR: Results indicate that both GC-IMS and GC-TOF-MS were able to discriminate PDAC from healthy controls with high confidence and an AUC in excess of 0.85, and chemical identification suggests that 2,6-dimethyl-octane, nonanal, 4-ethyl-1,2-dim methyl-benzene and 2-pentanone play an important role in separating these groups.
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Review and Comparison of Cancer Biomarker Trends in Urine as a Basis for New Diagnostic Pathways.
TL;DR: This investigation aims to evaluate the possibility to compare the results of studies based on different approaches to be able in the future to identify those compounds responsible for urine odor alteration and to highlight the correlation between urine odor alterations and cancer presence.
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