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Journal ArticleDOI

Use of Detergents in the Analysis of Fibrous Feeds. II. A Rapid Method for the Determination of Fiber and Lignin

01 Oct 1963-Journal of AOAC International (Oxford Academic)-Vol. 46, Iss: 4, pp 829-835
TL;DR: The acid-detergent fiber method (ADF) as mentioned in this paper is a fiber method based on cetyl trimethylammonium bromide to dissolve proteins in acid solution.
Abstract: The capacity of cetyl trimethylammonium bromide to dissolve proteins in acid solution has been utilized in development of a method, called acid-detergent fiber method (ADF), which is not only a fiber determination in itself bnt also the major preparatory step in the determination of lignin. The entire procedure for determining fiber and lignin is considerably more rapid than presently published methods. Compositional studies show ADF to consist chiefly of lignin and polysaccharides. Correlations with the new fiber method and digestibility of 18 forages (r = —0.79) showed it to be somewhat superior to crude fiber (r = —0.73) in estimating nutritive valué. The correla-
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Journal ArticleDOI
TL;DR: In addition to NDF, new improved methods for total dietary fiber and nonstarch polysaccharides including pectin and beta-glucans now are available and are also of interest in rumen fermentation.

23,302 citations

Journal ArticleDOI
01 Jun 1982-Ecology
TL;DR: The effects of initial nitrogen and lignin contents of six species of hardwood leaves on their decomposition dynamics were studied at the Hubbard Brook Experimental Forest by inverse linear relationships between the percentage of original mass remaining and the nitrogen concentration in the residual material.
Abstract: The effects of initial nitrogen and lignin contents of six species of hardwood leaves on their decomposition dynamics were studied at the Hubbard Brook Experimental Forest. Rate con- stants (k) for annual leaf mass loss ranged from -0.08 to -0.47. The rate constants (k) had a negative linear correlation (r2 = .89) with the ratio of initial lignin concentration to initial nitrogen concentra- tion. Decomposition dynamics of the litter materials were described by inverse linear relationships between the percentage of original mass remaining and the nitrogen concentration in the residual material. Initial lignin concentration was highly correlated (r2 = .93) with the slope of the inverse linear relationship for each of the litter types.

2,612 citations

Journal ArticleDOI
TL;DR: The Cornell Net Carbohydrate Protein Model (Chalupa et al., 1991) has developed the need for uniform procedures to partition feed nitrogen into A, B, and C fractions (Pichard and Van Soest, 1977) as mentioned in this paper.

2,282 citations

Journal ArticleDOI
TL;DR: It is proposed that labile plant constituents are the dominant source of microbial products, relative to input rates, because they are utilized more efficiently by microbes, and become the main precursors of stable SOM by promoting aggregation and through strong chemical bonding to the mineral soil matrix.
Abstract: The decomposition and transformation of above- and below-ground plant detritus (litter) is the main process by which soil organic matter (SOM) is formed. Yet, research on litter decay and SOM formation has been largely uncoupled, failing to provide an effective nexus between these two fundamental processes for carbon (C) and nitrogen (N) cycling and storage. We present the current understanding of the importance of microbial substrate use efficiency and C and N allocation in controlling the proportion of plant-derived C and N that is incorporated into SOM, and of soil matrix interactions in controlling SOM stabilization. We synthesize this understanding into the Microbial Efficiency-Matrix Stabilization (MEMS) framework. This framework leads to the hypothesis that labile plant constituents are the dominant source of microbial products, relative to input rates, because they are utilized more efficiently by microbes. These microbial products of decomposition would thus become the main precursors of stable SOM by promoting aggregation and through strong chemical bonding to the mineral soil matrix.

1,851 citations


Cites methods from "Use of Detergents in the Analysis o..."

  • ...The ‘lignin’ of the conventional model is an operationally defined fraction of litter that is not degraded by strong acid treatment, as measured by the acid detergent fiber method (Van Soest, 1963)....

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Journal ArticleDOI
TL;DR: In this paper, a total, soluble and insoluble non-starch polysaccharides (NSP) and lignin were analysed for low-molecular weight (LMW) sugars by high-performance liquid chromatography, starch, fructan and mixed linked β(1 → 3;1 → 4)-D-glucan by colorimetry, total, insoluble NSP by gas-liquid chromatography and Lignin by gravimetry.

1,449 citations

References
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Book
01 Jan 1952

346 citations