Use of MTT colorimetric assay to measure cell activation
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...Cell viability was tested using XTT (sodium 3′-[1-(phenylaminocarbonyl)-3,4tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfonic acid hydrate) assay (Gerlier and Thomasset, 1986) based on the cleavage of yellow tetrazolium salt XTT by metabolically active cells to form an orange formazan dye which was quantified using ELISA reader (Biorad, USA, model 680)....
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...According to Mosmann (1983) and Gerlier and Thomasset (1986), who demonstrated that the formazan generated would depend on cellular metabolism, this result may reflect the metabolic state of the cells, being worse 2 h after the splitting procedure than after 48 h culture....
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...…of formazan produced by MVE2-activated macrophages were similar to those of non-activated macrophages , is in contrast to the results obtained by Gerlier and Thomasset (1986), attesting to the general applicability of the MTT cleavage method for measuring cell activation irrespective 'of cell…...
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