Utilization of phosphorus by Streptomyces griseus during its aerobic and anaerobic growth
TL;DR: From the chromatograms of different phosphate esters present in the mycelial extracts of S. griseus, it appears that the Embden-Meyerhof cycle is operative during anaerobiosis, while some other pathway, possibly the hexose monophosphate shunt, proceeds during aerobic growth.
About: This article is published in Archives of Biochemistry and Biophysics.The article was published on 1956-07-01. It has received 4 citations till now. The article focuses on the topics: Phosphorus utilization & Phosphorus metabolism.
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TL;DR: A more complete characterization of the respiratory chain of the streptomyces is reported, not in accord with the spectroscopic observations of Inoue (1958), who detected a-, b-, and c-type cytochromes in mycelium of StrePTomyces griseus.
Abstract: There is currently a great deal of interest in the streptomyces as producers of antibiotics, but relatively little is known about the mechanisms of intracellular energy supply in this group of organisms. They are aerobes, and carbohydrates serve as an energy source. There is good evidence that the pathways of carbohydrate metabolism in the streptomyces include the glycolysis sequence (Cochrane, 1955), the pentose cycle reactions (Cochrane, Peck, and Harrison, 1953; Ganguly and Roy, 1956; Maitra and Roy, 1959), and the Krebs cycle (Cochrane and Peck, 1953; Nickerson and Ram Mohan, 1953; Ganguly and Roy, 1955). However, the pathway of hydrogen (electron) transfer to molecular oxygen has never been studied in detail. Sato (1940) first reported the presence of cytoehromes in the Actinomyeetes. On the basis of a spectroplhotometric survey of the cytochrome components in 13 Streptomyces species, Heim, Silver, and Birk (1957) concluded that a b-type cytochrome is always present, but in no case were they able to detect cytochrome a and in some species cytochrome c was also apparently missing. They suggested that these organisms may have an atypical respiratory chain that terminates with cytochrome b, and this view was subsequently supported by the partial purification of an autoxidizable b-component from extracts of Streptomyces fradiae (Birk, Silver, and Heim, 1957). These findings are not in accord with the spectroscopic observations of Inoue (1958), who detected a-, b-, and c-type cytochromes in mycelium of Streptomyces griseus. We have verified this observation using cell-free extracts of various streptomyces (Niederpruem and Hackett, 1959). The present paper reports a more complete characterization of the respiratory chain both
12 citations
TL;DR: The present investigation focuses on Streptomyces olivaceus, a species of the same genus that is exploited industrially for the production of vitamin Blz and shows that this organism utilizes both the glycolytic and the hexose monophosphate pathways for the metabolism of glucose.
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TL;DR: Modifications in Hanes and Isherwood's method for the separation of phosphorylated sugars and adenosine phosphates by paper chromatography similar to those being employed in this laboratory are indicated.
Abstract: Bandurski and Axelrod1 have recently published a paper describing modifications in Hanes and Isherwood's method2 for the separation of phosphorylated sugars and adenosine phosphates by paper chromatography. Since these modifications are in certain respects similar to those being employed in this laboratory, this opportunity is being taken of indicating briefly the methods in use here.
222 citations
TL;DR: In their fundamental researches, Warburg and his co-workers have described in detail the enzymatic oxidation of glucosemonophosphate to phosphogluconic acid and found that the oxidation product is easily fermented by yeast macerate.
Abstract: IN their fundamental researches, Warburg and his co-workers1 have described in detail the enzymatic oxidation of glucosemonophosphate to phosphogluconic acid. Furthermore, they found that the oxidation product is easily fermented by yeast macerate. Now, phosphogluconic acid might be a first product of carbohydrate oxidation occurring in a manner different from fermentative breakdown. Then the breakdown of phosphogluconic acid effected by yeast extract might represent a further step in the course of oxidation.
153 citations