Vanadate is a potent (Na,K)-ATPase inhibitor found in ATP derived from muscle.
Reads0
Chats0
TLDR
A potent (Na,K)-ATPase inhibitor purified from "Sigma Grade* ATP" has been identified as vanadium using electron probe microanalysis and confirmed by microwave-induced emission spectroscopy and electron paramagnetic resonanceSpectroscopy.About:
This article is published in Journal of Biological Chemistry.The article was published on 1977-11-10 and is currently open access. It has received 815 citations till now. The article focuses on the topics: Vanadate & Sodium orthovanadate.read more
Citations
More filters
Journal ArticleDOI
Inhibition of membrane phosphotyrosyl-protein phosphatase activity by vanadate.
TL;DR: Results show that vanadate is a potent and selective inhibitor of phosphotyrosyl-protein phosphatase.
Journal ArticleDOI
Functional comparisons between isoforms of the sarcoplasmic or endoplasmic reticulum family of calcium pumps.
TL;DR: These properties are consistent with an enzyme in which the equilibrium between the E1 and E2 conformations is shifted toward the E2 state.
Journal ArticleDOI
Cyclin activation of p34cdc2
TL;DR: The concerted transition into mitosis involves both a reduction in the rate of p34cdc2 phosphorylation on tyrosine and an increase in its rate of dephosphorylation.
Journal ArticleDOI
A circulating inhibitor of (Na+ + K+) ATPase associated with essential hypertension
John M. Hamlyn,Richard Ringel,Juergen Schaeffer,Paul Levinson,Bruce P. Hamilton,A. Avinoam Kowarski,Mordecai P. Blaustein +6 more
TL;DR: A highly significant correlation is demonstrated between levels of a plasma inhibitor of (Na+ + K+)ATPase activity and mean arterial blood pressure in normotensive and hypertensive individuals, providing evidence for the involvement of a circulating Na+ pump inhibitor in the genesis of essential hypertension.
Journal ArticleDOI
Insulin-like stimulation of glucose oxidation in rat adipocytes by vanadyl (IV) ions
TL;DR: Externally applied vanadate ions at low concentrations mimic fully the effect of insulin on glucose oxidation in rat adipocytes, however, this simulation seems to be due mainly to the effects of vandyl (IV) ions, probably produced within the cells, and not primarily to inhibition of the sodium pump.
References
More filters
Journal ArticleDOI
Purification and characterization of (Na+ + K+)-ATPase III. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate
TL;DR: Analysis of the protein composition by sodium dodecylsulphate gel electrophoresis and determination of the capacities for binding of ATP and ouabain and for sodium-dependent phosphorylation show that the procedures lead to a true purification of the enzyme.
Journal ArticleDOI
Isolation of a potent (Na -K) stimulated ATPase inhibitor from striated muscle
Lee Josephson,Lewis C. Cantley +1 more
Journal ArticleDOI
Vanadium ion inhibition of alkaline phosphatase-catalyzed phosphate ester hydrolysis
TL;DR: Observations indicate that VO3− can bind quite specifically at the phosphate-binding site on the enzyme, which suggests that the enzyme-complexed inhibitor may have some resemblance to the metastable intermediate formed during the hydrolysis of phosphate esters.