Volatilization of mercury from natural water by a broad-spectrum Hg-resistant Bacillus pasteurii strain DR2
TL;DR: A broad spectrum mercury-resistant bacterial strain was isolated from contaminated water and was identified as Bacillus pasteurii strain DR2 as mentioned in this paper, which could volatilize Hg-compounds including organomercurials from its growth media.
About: This article is published in The Environmentalist.The article was published on 1996-09-01. It has received None citations till now. The article focuses on the topics: Mercury (element).
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TL;DR: The authors report on enzymatic volatilization of HgCl{sub 2} by fourteen Hg-resistant bacterial strains and studied thiol specificity of HG-reductases and organomercurial lyases isolated from the above bacterial species.
Abstract: Plasmid-determined mercuric and organomercurial resistance in microorganisms has been studied by several workers. Mercury reductase, catalyzing the reduction of mercury depends on sulfhydryl compounds. Organomercurial lyase that catalyzes the splitting of C-Hg linkages also needs thiol compounds for its activity. Until recently, no study has been reported on thiol specificity of these enzymes from various sources. In the present study, the authors report on enzymatic volatilization of HgCl{sub 2} by fourteen Hg-resistant bacterial strains. They have also studied thiol specificity of Hg-reductases and organomercurial lyases isolated from the above bacterial species. Hg-reductase is known to have FAD-moiety which stimulates enzyme activity whereas FMN and riboflavin are ineffective in this regard. The effect of flavins, namely FAD, FMN and riboflavin, on Hg-reductase and organomercurial lyase activity is also reported here.
14 citations
"Volatilization of mercury from natu..." refers background or methods in this paper
...HgC12 was added to induce mercuric reductase and organo-mercurial lyase ( Pahan et al., 1990a ) and benzene was added to stimulate its growth (Pahan et al., 1993a)....
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...A broad-spectrum Hg-resistant Bacillus pasteurii strain DR2 that volatilized Hg-compounds including organo-mercurials from its growth media and utilized different aromatic compounds as a sole source of carbon has been isolated ( Pahan et al., 1990a; Pahan et al., 1991a)....
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TL;DR: The author isolated a broad-spectrum Hg-resistant Bacillus pasteurii strain DR{sub 2} which could volatilize different mercury compounds and utilize various aromatic compounds as sole sources of carbon and preferentially utilized benzene in a medium containing both glucose and benzene.
Abstract: Aquatic ecosystems may receive aromatic compounds through various routes These compounds can cause cancerous diseases in aquatic animals and enhance mutagenicity of the sediments The persistence of aromatic compounds deposited in sediments is affected by microbial degradation Plasmid-determined mercuric and organomercurial resistance in microorganisms has also been studied by several workers Utilization of various aromatic compounds as sole sources of carbon by an Hg-resistant bacterial strain has not been reported The author isolated a broad-spectrum Hg-resistant Bacillus pasteurii strain DR{sub 2} which could volatilize different mercury compounds and utilize various aromatic compounds as sole sources of carbon This strain preferentially utilized benzene in a medium containing both glucose and benzene To their knowledge, until recently there has been no report on preferential utilization of other compounds, particularly an aromatic compound to glucose in a mixture
10 citations
TL;DR: A large number of mercury-resistant nitrogen-fixing bacteria were isolated by screening soil samples from agricultural farms of West Bengal, India and identified to the generic level following Bergey's Manual of Determinative Bacteriology, 8th edition.
Abstract: A large number of mercury-resistant nitrogen-fixing bacteria were isolated by screening soil samples from agricultural farms of West Bengal, India. All of the samples were found to contain significant quantities of mercury. Twenty five of the pure isolates were identified to the generic level following Bergey's Manual of Determinative Bacteriology, 8th edition. These organisms were resistant to HgCl2, and a few of them were resistant to phenylmercuric acetate (PMA) as well. Their mercury resistance was also associated with antibiotic resistance properties. Growth of two mercury-resistant N2-fixing organisms was monitored in liquid media supplemented with HgCl2. The mercury volatilizing capacity of four mercury-resistant bacterial strains was also determined.
10 citations
TL;DR: Inhibition of the glucose oxidation rate by these chemicals in lysozyme-treated cells indicated that these compounds facilitated the transport of glucose across the cell wall and thereby stimulated growth.
Abstract: A broad spectrumbroad spectrum mercury-resistant organism, Bacillus pasteurii DR 2 , was isolated from the effluents of Durgapur Steel Plant, India. In the presence of phenylmercuric acetate or benzene the rate of glucose uptake and the level of some metabolic enzymes increased significantly. Inhibition of the glucose oxidation rate by these chemicals in lysozyme-treated cells indicated that these compounds facilitated the transport of glucose across the cell wall and thereby stimulated growth
7 citations
"Volatilization of mercury from natu..." refers background or methods in this paper
...Even the bacterial strain survived better in the presence of PMA than in the presence of HgCI2 which may be due to the facilitated transport of nutrients through the cell membrahe ( Pahan et al., 1993a )....
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...Its growth was also stimulated in the presence of benzene and phenylmercuric acetate ( Pahan et al., 1993a )....
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...HgC12 was added to induce mercuric reductase and organo-mercurial lyase (Pahan et al., 1990a) and benzene was added to stimulate its growth ( Pahan et al., 1993a )....
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4 citations
"Volatilization of mercury from natu..." refers background in this paper
...This strain can also degrade and utilize fluorescein, mercuric acetate and merbromine ( Pahan et al., 1992 )....
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