What Are the Functions of Chitin Deacetylases in Aspergillus fumigatus
Isabelle Mouyna,Sarah Dellière,Anne Beauvais,Fabrice N. Gravelat,Fabrice N. Gravelat,Brendan D. Snarr,Brendan D. Snarr,Melanie Lehoux,Caitlin A. Zacharias,Caitlin A. Zacharias,Yan Sun,Steven de Jesus Carrion,Eric Pearlman,Donald C. Sheppard,Donald C. Sheppard,Jean-Paul Latgé +15 more
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TLDR
The results suggest that chitosan is a minority component of the A. fumigatus cell wall, and that the seven candidate Cda proteins do not play major roles in fungal cell wall synthesis or virulence.Abstract:
Deacetylation of chitin by chitin deacetylases (Cda) results in the formation of chitosan. Chitosan, a polymer of β1,4 linked glucosamine, plays multiple roles in the function of the fungal cell wall, including virulence and evasion of host immune responses. In this study, the roles of chitosan and putative CDAs in cell wall structure and virulence of Aspergillus fumigatus were investigated. Low levels of chitosan were found in the conidial and cell wall of A. fumigatus. Seven putative CDA genes were identified, disrupted and the phenotype of the single mutants and the septuple mutants were investigated. No alterations in fungal cell wall chitosan levels, changes in fungal growth or alterations in virulence were detected in the single or septuple Δcda1-7 mutant strains. Collectively, these results suggest that chitosan is a minority component of the A. fumigatus cell wall, and that the seven candidate Cda proteins do not play major roles in fungal cell wall synthesis or virulence. However, Cda2 is involved in conidiation, suggesting that this enzyme may play a role in N-acetyl-glucosamine metabolism.read more
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Structural Polymorphism of Chitin and Chitosan in Fungal Cell Walls From Solid-State NMR and Principal Component Analysis.
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Chitosan and Chitin Deacetylase Activity Are Necessary for Development and Virulence of Ustilago maydis.
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FastTree: Computing Large Minimum Evolution Trees with Profiles instead of a Distance Matrix
TL;DR: FastTree is a method for constructing large phylogenies and for estimating their reliability, instead of storing a distance matrix, that uses sequence profiles of internal nodes in the tree to implement Neighbor-Joining and uses heuristics to quickly identify candidate joins.
Journal Article
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TL;DR: FastTree as mentioned in this paper uses sequence profiles of internal nodes in the tree to implement neighbor-joining and uses heuristics to quickly identify candidate joins, then uses nearest-neighbor interchanges to reduce the length of the tree.
Journal ArticleDOI
Negative magnetoresistance without well-defined chirality in the Weyl semimetal TaP
Frank Arnold,Chandra Shekhar,Shu-Chun Wu,Yan Sun,Ricardo Donizeth dos Reis,Nitesh Kumar,Marcel Naumann,M. O. Ajeesh,Marcus Schmidt,Adolfo G. Grushin,Jens H. Bardarson,Michael Baenitz,Dmitry A. Sokolov,Horst Borrmann,Michael Nicklas,Claudia Felser,Elena Hassinger,Binghai Yan +17 more
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