wzi Gene Sequencing, a Rapid Method for Determination of Capsular Type for Klebsiella Strains
Sylvain Brisse,Sylvain Brisse,Virginie Passet,Virginie Passet,Anita Björk Haugaard,Anamaria Babosan,Najiby Kassis-Chikhani,Carsten Struve,Dominique Decré +8 more
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TLDR
Wzi sequencing is a rapid and simple method for the determination of the K types of most K. pneumoniae clinical isolates and the prediction of K type once the wzi allele was known was 94%.Abstract:
Pathogens of the genus Klebsiella have been classified into distinct capsular (K) types for nearly a century. K typing of Klebsiella species still has important applications in epidemiology and clinical microbiology, but the serological method has strong practical limitations. Our objective was to evaluate the sequencing of wzi, a gene conserved in all capsular types of Klebsiella pneumoniae that codes for an outer membrane protein involved in capsule attachment to the cell surface, as a simple and rapid method for the prediction of K type. The sequencing of a 447-nucleotide region of wzi distinguished the K-type reference strains with only nine exceptions. A reference wzi sequence database was created by the inclusion of multiple strains representing K types associated with high virulence and multidrug resistance. A collection of 119 prospective clinical isolates of K. pneumoniae were then analyzed in parallel by wzi sequencing and classical K typing. Whereas K typing achieved typeability for 81% and discrimination for 94.4% of the isolates, these figures were 98.1% and 98.3%, respectively, for wzi sequencing. The prediction of K type once the wzi allele was known was 94%. wzi sequencing is a rapid and simple method for the determination of the K types of most K. pneumoniae clinical isolates.read more
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Klebsiella pneumoniae: Going on the Offense with a Strong Defense
Michelle K. Paczosa,Joan Mecsas +1 more
TL;DR: Recent studies have identified additional K. pneumoniae virulence factors and led to more insights about factors important for the growth of this pathogen at a variety of tissue sites, but much work is left to be done in characterizing these newly discovered factors.
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Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health
Kathryn E. Holt,Heiman F. L. Wertheim,Ruth N. Zadoks,Stephen Baker,Chris A. Whitehouse,David A. B. Dance,Adam Jenney,Thomas R. Connor,Li Yang Hsu,Juliëtte A. Severin,Sylvain Brisse,Hanwei Cao,Jonathan J. Wilksch,Claire L. Gorrie,Mark B. Schultz,David J. Edwards,Kinh Van Nguyen,Trung Vu Nguyen,Trinh Tuyet Dao,Martijn Mensink,Vien Le Minh,Nguyen Thi Khanh Nhu,Constance Schultsz,Kuntaman Kuntaman,Paul N. Newton,Paul N. Newton,Catrin E. Moore,Catrin E. Moore,Richard A. Strugnell,Nicholas R. Thomson,Nicholas R. Thomson +30 more
TL;DR: The DNA sequence of K. pneumoniae isolates from around the world is determined and it is shown that there is a wide spectrum of diversity, including variation within shared sequences and gain and loss of whole genes, and there is an unrecognized association between the possession of specific gene profiles associated with virulence and antibiotic resistance.
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Colonization, Infection, and the Accessory Genome of Klebsiella pneumoniae
TL;DR: This review will explore the associations between colonization and infection with opportunistic, antibiotic-resistant, and hypervirulent K. pneumoniae strains and the role of the accessory genome in distinguishing these groups and related species.
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Identification of Klebsiella capsule synthesis loci from whole genome data.
Kelly L. Wyres,Ryan R. Wick,Claire L. Gorrie,Adam Jenney,Rainer Follador,Nicholas R. Thomson,Kathryn E. Holt +6 more
TL;DR: Kaptive, a novel software tool that automates the process of identifying K-loci based on full locus information extracted from whole genome sequences, is introduced, highlighting the extensive diversity of Klebsiella K- loci and the proteins that they encode.
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