Zinc-morin complex improves pancreatic β-cell function in the HFD-STZ induced experimental type 2 diabetes
01 Jan 2015-Vol. 7, Iss: 5, pp 1-10
TL;DR: Oral administration of zinc-morin complex at a concentration of 5mg/kg body weight to diabetic rats for a period of 30 days resulted in significant improvement in pancreatic β-cellfunction, which in turn may be due to the increased level of metallothionein, a reservoir of zinc.
Abstract: Hyperglycemia induced oxidative stress plays a pivotal role in the initiation and progression of diabetes and its secondary complications. An increase in oxidative damage due to excessive generation of free radicals is associated with etiology of insulin resistance. The sensitivity of pancreatic β-cells to oxidative stress has been attributed to their low content of antioxidants compared with other tissues. Recently, we have synthesized, characterized a new zinc-morin complex and evaluated its antidiabetic potentialin HFD fed-low dose STZ induced type 2 diabetic rats. The present study was aimed to study the role of zinc-morin complex on hyperglycemia mediated oxidative stress in diabetic rats. Oral administration zinc-morin complex at a concentration of 5mg/kg body weight to diabetic rats for a period of 30 days resulted in significant improvement in pancreatic β-cellfunction, which in turn may be due to the increased level of metallothionein, a reservoir of zinc. Further, treatment with zinc-morin complex significantly improved the levels of enzymaticand non-enzymatic antioxidants and reduced the levels of lipid peroxides, nitric oxide, IL-1β and nuclear NF-κB p65 unit in pancreatic tissues of diabetic rats. Ultrastructural observationsfurther evidenced the pancreaticβ-cell protective nature of zinc-morin complex against oxidative damage.
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TL;DR: Liraglutide significantly improved the symptoms of high-fat-fed and streptozotocin-induced type 2 diabetic mice, as indicated by body weight gain, reduction of blood glucose and plasma insulin, and enhanced sensitivity to insulin.
Abstract: BACKGROUND Clinical and experimental studies have revealed that liraglutide has multiple anti-diabetes biological effects. However, little is known about its role in autophagy and pancreatic β cell proliferation. This study aimed to assessed the effects of liraglutide on pancreatic b cell proliferation and autophagy in a mouse model of type 2 diabetes. MATERIAL AND METHODS The effect of liraglutide on autophagy and proliferation in pancreatic β cells was investigated using a high-fat-fed and streptozotocin-induced mouse model of type 2 diabetes. RESULTS Liraglutide significantly improved the symptoms of high-fat-fed (HFD) and streptozotocin (STZ)-induced type 2 diabetic mice, as indicated by body weight gain, reduction of blood glucose and plasma insulin, and enhanced sensitivity to insulin. The results of quantitative real-time polymerase chain reaction and Western blot analysis showed that liraglutide upregulated AGT5 expression and promoted the conversion of LC3-I to LC3-II, thus improving the defective autophagy. In addition, we observed that both mRNA and protein expressions of PCNA and Ki-67 were upregulated by liraglutide treatment. Immunocytochemical staining results showed that the number of PCNA- or Ki-67-positive cells in pancreatic islet tissues in the HFD + STZ + liraglutide group were increased compared with the HFD + STZ group. CONCLUSIONS These results strongly suggest that liraglutide is able to enhance autophagy and promote pancreatic β cell proliferation. This study improves our insights into the mechanism by which liraglutide treatment relieves diabetes, and provides experimental evidence for clinical utilization of liraglutide in type 2 diabetes treatment.
25 citations
Cites background from "Zinc-morin complex improves pancrea..."
...Many studies have demonstrated that HFD and STZ-treated mice have elevated glucose and plasma insulin levels and impaired ability to maintain glucose homeostasis, as well as weakened autophagy and pancreatic b cell apoptosis [4,10,11]....
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TL;DR: In this paper, metal morin nanocomposites have been used for the sensing of methylmercury at pM concentrations and it was found that the morin nano-composites showed a selective optical response towards methylmerscury by generating an absorbance peak at λmax 250'nm.
Abstract: In this article we report on the synthesis of metal morin nanocomposites. Morin, a naturally occurring polyphenol has been used to generate nickel, copper and nickel-copper bimetallic nanocomposites at room temperature. The method offers advantage of being simple and free from environmental hazards. The synthesized nanocomposites have been characterized thoroughly using various analytical techniques. The materials so obtained were explored for the sensing of methylmercury at pM concentrations. It was found that the morin nanocomposites showed selective optical response towards methylmercury by generating an absorbance peak at λmax 250 nm. Out of the three nanocomposites, Cu- morin nanocomposite was found to be a better candidate as sensor. The linear range of detection was found to be 33 pM–100 pM. Infra red spectroscopic data suggested formation of Hg C bond as the reason behind such type of sensing. This method was further extended towards detection of methylmercury in the natural samples like, river water and its sediment. The material can be reused with significant efficiency for two successive runs.
3 citations
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TL;DR: Liraglutide significantly improved the symptoms of high-fat-fed and streptozotocin-induced type 2 diabetic mice, as indicated by body weight gain, reduction of blood glucose and plasma insulin, and enhanced sensitivity to insulin.
Abstract: BACKGROUND Clinical and experimental studies have revealed that liraglutide has multiple anti-diabetes biological effects. However, little is known about its role in autophagy and pancreatic β cell proliferation. This study aimed to assessed the effects of liraglutide on pancreatic b cell proliferation and autophagy in a mouse model of type 2 diabetes. MATERIAL AND METHODS The effect of liraglutide on autophagy and proliferation in pancreatic β cells was investigated using a high-fat-fed and streptozotocin-induced mouse model of type 2 diabetes. RESULTS Liraglutide significantly improved the symptoms of high-fat-fed (HFD) and streptozotocin (STZ)-induced type 2 diabetic mice, as indicated by body weight gain, reduction of blood glucose and plasma insulin, and enhanced sensitivity to insulin. The results of quantitative real-time polymerase chain reaction and Western blot analysis showed that liraglutide upregulated AGT5 expression and promoted the conversion of LC3-I to LC3-II, thus improving the defective autophagy. In addition, we observed that both mRNA and protein expressions of PCNA and Ki-67 were upregulated by liraglutide treatment. Immunocytochemical staining results showed that the number of PCNA- or Ki-67-positive cells in pancreatic islet tissues in the HFD + STZ + liraglutide group were increased compared with the HFD + STZ group. CONCLUSIONS These results strongly suggest that liraglutide is able to enhance autophagy and promote pancreatic β cell proliferation. This study improves our insights into the mechanism by which liraglutide treatment relieves diabetes, and provides experimental evidence for clinical utilization of liraglutide in type 2 diabetes treatment.
25 citations
TL;DR: In this paper, metal morin nanocomposites have been used for the sensing of methylmercury at pM concentrations and it was found that the morin nano-composites showed a selective optical response towards methylmerscury by generating an absorbance peak at λmax 250'nm.
Abstract: In this article we report on the synthesis of metal morin nanocomposites. Morin, a naturally occurring polyphenol has been used to generate nickel, copper and nickel-copper bimetallic nanocomposites at room temperature. The method offers advantage of being simple and free from environmental hazards. The synthesized nanocomposites have been characterized thoroughly using various analytical techniques. The materials so obtained were explored for the sensing of methylmercury at pM concentrations. It was found that the morin nanocomposites showed selective optical response towards methylmercury by generating an absorbance peak at λmax 250 nm. Out of the three nanocomposites, Cu- morin nanocomposite was found to be a better candidate as sensor. The linear range of detection was found to be 33 pM–100 pM. Infra red spectroscopic data suggested formation of Hg C bond as the reason behind such type of sensing. This method was further extended towards detection of methylmercury in the natural samples like, river water and its sediment. The material can be reused with significant efficiency for two successive runs.
3 citations