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/Title (Detection\040of\0402019\040novel\040coronavirus\040\0502019\055nCoV\051\040by\040real\055time\040RT\055PCR\056)
/Author (Victor\040M\056\040Corman\054\040Olfert\040Landt\054\040Marco\040Kaiser\054\040Richard\040Molenkamp\054\040Adam\040Meijer\054\040Daniel\040K\056W\056\040Chu\054\040Tobias\040Bleicker\054\040Sebastian\040Brnink\054\040Julia\040Schneider\054\040Marie\040Luisa\040Schmidt\054\040Daphne\040G\056J\056C\056\040Mulders\054\040Bart\040L\056\040Haagmans\054\040Bas\040van\040der\040Veer\054\040Sharon\040van\040den\040Brink\054\040Lisa\040Wijsman\054\040Gabriel\040Goderski\054\040Jean\040Louis\040Romette\054\040Joanna\040Ellis\054\040Maria\040Zambon\054\040Malik\040Peiris\054\040Herman\040Goossens\054\040Chantal\040B\056E\056M\056\040Reusken\054\040Marion\040Koopmans\054\040Christian\040Drosten)
/Subject (Background\040The\040ongoing\040outbreak\040of\040the\040recently\040emerged\040novel\040coronavirus\040\0502019\055nCoV\051\040poses\040a\040challenge\040for\040public\040health\040laboratories\040as\040virus\040isolates\040are\040unavailable\040while\040there\040is\040growing\040evidence\040that\040the\040outbreak\040is\040more\040widespread\040than\040initially\040thought\054\040and\040international\040spread\040through\040travellers\040does\040already\040occur\056\040Aim\040We\040aimed\040to\040develop\040and\040deploy\040robust\040diagnostic\040methodology\040for\040use\040in\040public\040health\040laboratory\040settings\040without\040having\040virus\040material\040available\056\040Methods\040Here\040we\040present\040a\040validated\040diagnostic\040workflow\040for\0402019\055nCoV\054\040its\040design\040relying\040on\040close\040genetic\040relatedness\040of\0402019\055nCoV\040with\040SARS\040coronavirus\054\040making\040use\040of\040synthetic\040nucleic\040acid\040technology\056\040Results\040The\040workflow\040reliably\040detects\0402019\055nCoV\054\040and\040further\040discriminates\0402019\055nCoV\040from\040SARS\055CoV\056\040Through\040coordination\040between\040academic\040and\040public\040laboratories\054\040we\040confirmed\040assay\040exclusivity\040based\040on\040297\040original\040clinical\040specimens\040containing\040a\040full\040spectrum\040of\040human\040respiratory\040viruses\056\040Control\040material\040is\040made\040available\040through\040European\040Virus\040Archive\040\205\040Global\040\050EVAg\051\054\040a\040European\040Union\040infrastructure\040project\056\040Conclusion\040The\040present\040study\040demonstrates\040the\040enormous\040response\040capacity\040achieved\040through\040coordination\040of\040academic\040and\040public\040laboratories\040in\040national\040and\040European\040research\040networks\056)
/Keywords (23\040Jan\0402020\054\040Eurosurveillance\054\040European\040union\054\040Charit\054\040Erasmus\040University\040Rotterdam\054\040University\040of\040Hong\040Kong\054\040Public\040Health\040England\054\040University\040of\040Antwerp)
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